Glutathione S-transferase P1 gene polymorphisms and susceptibility to coronary artery disease in a subgroup of north Indian population

Abstract

The present study aimed to investigate the association of $\hbox {g}.313\hbox {A}{>}\hbox {G}$ and $\hbox {g}.341\hbox {C}{>}\hbox {T}$ polymorphisms of GSTP1 with coronary artery disease (CAD) in a subgroup of north Indian population. In the present case–control study, CAD patients ($n = 200$) and age-matched, sex-matched and ethnicity-matched healthy controls ($n = 200$) were genotyped for polymorphisms in GSTP1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genotype distribution of $\hbox {g}.313\hbox {A}{>}\hbox {G}$ and $\hbox {g}.341\hbox {C}{>}\hbox {T}$ polymorphisms of GSTP1 gene was significantly different between cases and controls ($P = 0.005$ and 0.024, respectively). Binary logistic regression analysis showed significant association of A/G (odds ratio (OR): 1.6, 95% CI: 1.08–2.49, $P = 0.020$) and G/G (OR: 3.1, 95% CI: 1.41–6.71, P $=$ 0.005) genotypes of GSTP1 $\hbox {g}.313\hbox {A}{\!>\!}\hbox {G}$, and C/T (OR: 5.8, 95% CI: 1.26–26.34, $P = 0.024$) genotype of GSTP1 $\hbox {g}.341\hbox {C}{>}\hbox {T}$ with CAD. The A/G and G/G genotypes of $\hbox {g}.313\hbox {A}{>}\hbox {G}$ and C/T genotype of $\hbox {g}.341\hbox {C}{>}\hbox {T}$ conferred 6.5-fold increased risk for CAD (OR: 6.5, 95% CI: 1.37–31.27, $P = 0.018$). Moreover, the recessive model of GSTP1 $\hbox {g}.313\hbox {A}{>}\hbox {G}$ is the best fit inheritance model to predict the susceptible gene effect (OR: 2.3, 95% CI: 1.11–4.92, $P = 0.020$). In conclusion, statistically significant associations of GSTP1 $\hbox {g}.313\hbox {A}{>}\hbox {G}$ (A/G, G/G) and $\hbox {g}.341\hbox {C}{>}\hbox {T}$ (C/T) genotypes with CAD were observed.