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Fluorescence microscopy applied to intracellular transport by microtubule motors

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Abstract

Long-distance transport of many organelles inside eukaryotic cells is driven by the dynein and kinesin motors on microtubule filaments. More than 30 years since the discovery of these motors, unanswered questions include motor–organelle selectivity, structural determinants of processivity, collective behaviour of motors and track selection within the complex cytoskeletal architecture, to name a few. Fluorescence microscopy has been invaluable in addressing some of these questions. Here we present a review of some efforts to understand these sub-microscopic machines using fluorescence.

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Acknowledgements

RM acknowledges funding from a Wellcome Trust International Senior Research Fellowship (grant WT079214MA) and a Senior fellowship from the Wellcome Trust – Department of Biotechnology, India, alliance (grant IA/S/11/2500255). We apologize to our colleagues whose work we could not discuss due to lack of space.

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  1. Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, 400005, India

    Divya Pathak & Roop Mallik

  2. Department of Physiology, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Shreyasi Thakur

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  1. Divya Pathak
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Pathak, D., Thakur, S. & Mallik, R. Fluorescence microscopy applied to intracellular transport by microtubule motors. J Biosci 43, 437–445 (2018). https://doi.org/10.1007/s12038-018-9765-2

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