Abstract
Major depressive disorder (MDD) is a severe disorder that causes enormous loss of quality of life, and among the factors underlying MDD is stress in maternal deprivation (MD). In addition, classic pharmacotherapy has presented severe adverse effects. Centella asiatica (C. asiatica) demonstrates a potential neuroprotective effect but has not yet been evaluated in MD models. This study aimed to evaluate the effect of C. asiatica extract and the active compound madecassic acid on possible depressive-like behavior, inflammation, and oxidative stress in the hippocampus and serum of young rats submitted to MD in the first days of life. Rats (after the first day of birth) were separated from the mother for 3 h a day for 10 days. When adults, these animals were divided into groups and submitted to treatment for 14 days. After subjecting the animals to protocols of locomotor activity in the open field and behavioral despair in the forced swimming test, researchers then euthanized the animals. The hippocampus and serum were collected and analyzed for the inflammatory cytokines and oxidative markers. The C. asiatica extract and active compound reversed or reduced depressive-like behaviors, inflammation in the hippocampus, and oxidative stress in serum and hippocampus. These results suggest that C. asiatica and madecassic acid have potential antidepressant action, at least partially, through anti-inflammatory and antioxidant profiles.
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Data Availability
The data that support the findings of this study are available on request from the corresponding author.
References
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Acknowledgements
Zuleide Maria Ignácio and Margarete Dulce Bagatini are supported by research grants from the National Council for Scientific and Technological Development (CNPq), Santa Catarina State Research and Innovation Support Foundation—FAPESC, and Federal University of Southern Frontier—UFFS.
Funding
This research was supported by grants from CNPq (ZMI and MDB), FAPESC (ZMI and MDB), and UFFS (ZMI and MDB).
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All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Amanda Gollo Bertollo, Maiqueli Eduarda Dama Mingoti, Jesiel de Medeiros, Gilnei Bruno da Silva, Giovana Tamara Capoani, Heloisa Lindemann, Joana Cassol, Daiane Manica, Tacio de Oliveira, Michelle Lima Garcez, Margarete Dulce Bagatini, Lilian Caroline Bohnen, Walter Antônio Roman Junior, and Zuleide Maria Ignácio. Amanda Gollo Bertollo wrote the first draft of the manuscript. All authors commented on the previous version of the manuscript. Zuleide Maria Ignácio reviewed and supervised the project. All authors read and approved the final manuscript.
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Bertollo, A.G., Mingoti, M.E.D., de Medeiros, J. et al. Hydroalcoholic Extract of Centella asiatica and Madecassic Acid Reverse Depressive-Like Behaviors, Inflammation and Oxidative Stress in Adult Rats Submitted to Stress in Early Life. Mol Neurobiol (2024). https://doi.org/10.1007/s12035-024-04198-1
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DOI: https://doi.org/10.1007/s12035-024-04198-1