Abstract
Neuroblasts were first derived from the adult mammalian brains in the 1990s by Reynolds et al. Since then, persistent neurogenesis in the subgranular zone (SGZ) of the hippocampus and subventricular zone (SVZ) has gradually been recognized. To date, reviews on neuroblast migration have largely investigated glial cells and molecular signaling mechanisms, while the relationship between vasculature and cell migration remains a mystery. Thus, this paper underlines the partial biological features of neuroblast migration and unravels the significance and mechanisms of the vasculature in the process to further clarify theoretically the neural repair mechanism after brain injury. Neuroblast migration presents three modes according to the characteristics of cells that act as scaffolds during the migration process: gliophilic migration, neurophilic migration, and vasophilic migration. Many signaling molecules, including brain-derived neurotrophic factor (BDNF), stromal cell-derived factor 1 (SDF-1), vascular endothelial growth factor (VEGF), and angiopoietin-1 (Ang-1), affect vasophilic migration, synergistically regulating the migration of neuroblasts to target areas along blood vessels. However, the precise role of blood vessels in the migration of neuroblasts needs to be further explored. The in-depth study of neuroblast migration will most probably provide theoretical basis and breakthrough for the clinical treatment of brain injury diseases.
Graphical Abstract
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Traumatic brain injury (TBI) has high mortality and morbidity, leading to severe neurological dysfunction [1, 2]. Neurogenesis involving the maintenance and self-renewal of neural stem cells (NSCs), as well as the survival, migration, maturation, and integration of neuroblasts [1], has underlined therapeutic options for treating TBI. Neuroblasts are immature cells of neuronal lineage that migrate to target brain regions from their birthplaces to become neurons and integrate into neural networks [3]. Neuroblasts were first derived from the adult mammalian brains in the 1990s by Reynolds et al. [4]. Since then, persistent neurogenesis in the subgranular zone (SGZ) of the hippocampus and subventricular zone (SVZ) has gradually been recognized [5,6,7,8,9,10,11,12]. Endogenous neurogenesis following brain injury occurs as follows [13,14,15]: neuroblasts in the SVZ migrate toward the damaged tissue and contribute to neuronal repair, which could be a lengthy process with multiple influencing factors [16,17,18,19,20].
Two distinct modes of neuroblast migration have been well recognized so far based on the direction of cell migration [18]: radial and tangential migration. However, neuroblast migration can be classified into neuronal migration, glial cell migration, and vascular migration, according to the medium of cell migration. To date, reviews on neuroblast migration have largely investigated glial cells and molecular signaling mechanisms, while the relationship between vasculature and cell migration remains a mystery. Thus, this paper underlines the partial biological features of neuroblast migration and unravels the significance and mechanisms of the vasculature in the process to further clarify theoretically the neural repair mechanism after brain injury and provide reference for clinical treatment of this diseased condition.
Traumatic Brain Injury
Pathological Mechanisms and Therapy of Traumatic Brain Injury (Fig. 1)
TBI can result from exposure to a blow or blast, rapid head deceleration or acceleration, and skull penetration, causing not a single pathophysiological event at the time of injury but a complex continuous disease process [21, 22]. After TBI, structural damage and functional deficits occur due to both primary and secondary injury mechanisms [2, 21]. Primary injury of mechanical tissue deformation and injury not only leads to cell death, shearing, and tearing of blood vessels, neuron, glia, and axon, but also initiates secondary injury cascades, such as excitotoxicity and oxidative stress. Excitotoxicity is nonspecific depolarization and release of excitatory neurotransmitters, glutamate, and aspartate, which bind to glutamate receptors and induce massive influx of calcium called as calcium overload. Calcium overload activates calcium-dependent phospholipases, proteases, and endonucleases, damaging cell membrane, cytoskeleton, and nucleic acids, respectively. Mitochondria sequester intracellular calcium may cause mitochondrial permeability pore opening, energy deficits, free radical formation, and initiation of apoptosis. Also, TBI initiates oxidative stress because of significantly increased formation of oxygen and nitrogen reactive species, which oxidize lipids, proteins, and nuclei acids. Furthermore, TBI upregulates transcription factors, inflammatory mediators, and neuroprotective genes but downregulates neurotransmitter receptors and neurotransmitter release mechanisms. Increased expression of detrimental cytokines and chemokines induces brain edema, blood–brain barrier damage, and apoptosis. These complex cascades subsequently induce blood–brain barrier damage, hemorrhage, edema, increased ICP, altered cerebral flow, ischemia/hypoxia, metabolic deficits, apoptosis, diffuse axonal injury, demyelination, progressive atrophy of both grey and white matter, which collectively cause cell death, brain neurodegeneration, and functional deficits. However, accumulative experimental and clinical data over the past decade have indicated that the adult brain is capable of, limited though, structural and functional reorganization after injury, possibly, contributing to spontaneous functional recovery. Recent new interventions targeting multiple secondary injury mechanisms and promoting neuroplasticity mechanisms have improved functional recovery in animal models of TBI.
Effective therapeutic strategies for TBI are lacking due to its heterogeneous nature [2, 21, 22]. Two strategic approaches have been developed: neuroprotective treatment and neurorestorative treatment [21]. Neuroprotective treatment targets the injured brain to reduce/prevent secondary injury and neural cell death, as well as reduce the lesion size; neurorestorative treatment improves neurological recovery by treating the entire central nervous system (CNS) to promote neurovascular remodeling including angiogenesis, neurogenesis, oligodendrogenesis, and dendrite/axon outgrowth [1, 2, 21]. However, ideal treatment for TBI remains to be further explored due to its multiple complications during pathogenesis.
Neuronal Apoptosis After Traumatic Brain Injury (Fig. 2)
Neuronal apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. Biochemical events lead to characteristic cell changes (morphology) and cell death [22]. These morphological changes include cell blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and messenger RNA decay. Triggers of apoptosis include oxygen free radicals, death receptor ligation, DNA damage, protease activation, and ionic imbalance. Both extrinsic (Fas and other tumor necrosis factor receptor superfamily members and ligands) and intrinsic (mitochondria-associated) pathways involved in apoptosis are found in the cytoplasm. The extrinsic pathway is triggered by death receptor engagement, initiating a signaling cascade mediated by caspase-8 activation, whereas the intrinsic pathway is engaged when various apoptotic stimuli trigger the release of cytochrome c from mitochondria independently of caspase-8 activation. Both pathways ultimately cause caspase-3 activation, degrading cellular proteins necessary to maintain cell survival and integrity. Besides, there is a complex interplay of the Bcl-2 family of proteins, which either promote (Bax, Bak, Bad, Bim, Bid) or prevent (Bcl-2, Bcl-xL, Bcl-w) injury. Bcl-2 and its family member, Bcl-xL, are among the most powerful death-suppressing proteins which inhibit both caspase-dependent and caspase-independent cell death. Apoptosis-inducing factor (AIF), a caspase-independent apoptotic pathway, is stored within the same mitochondrial compartment as cytochrome c. DNA damage via PARP activation and oxidative or excitotoxic stress release AIF, which is translocated to the nucleus to induce apoptosis. Figure 2 shows these pathways.
Neurogenesis in Adult Brain
Neurogenesis involves the maintenance and self-renewal of neural stem cells (NSCs), as well as the survival, migration, maturation, and integration of neuroblasts. The SGZ of the hippocampus and the SVZ are regions where adult neurogenesis mostly occurs [23, 24], the latter of which mainly contains the following architectures (Fig. 3): ependymal cells; type B1 cells, i.e., SVZ stem cells; type C cells, i.e., rapidly proliferating neuroblasts; and type A cells, i.e., migratory neuroblasts. Type B1 cells can exist in the quiescent state, with most of the cells being GFAP-positive, or in an activated state, being Nestin-positive. When activated, type B1 cells undergo asymmetrical division and turn into type C cells, which rapidly proliferates into DCX-positive, migratory type A cells [25]. Under normal circumstances, type A neuroblasts in the rostral migratory stream (RMS) migrate to the olfactory bulb (OB); in the event of brain injury, part of the neuroblasts move out of the RMS and migrate toward the focal area [23, 26].
Cellular Mechanisms of Neuroblast Migration
Neuroblast migration, although diverse in modes and pathways, undergo three cytological events [27,28,29]: extension of the leading process, which has a growth cone at its distal tip to explore microenvironment; forward movement of the centrosome, and translocation of the nucleus, i.e., “nucleokinesis”; trailing process retraction. Recurrence of the above three events contributes to the overall movement of the neuron.
Marin et al. [19] elaborated three events of neuroblast migration and their influencing molecules (Fig. 4). First, in the extension of the leading process, the PI3K signaling pathway plays a significant role, with RhoA, Rac1, and Cdc42 functioning as three critical regulatory molecules in this pathway. Inhibiting RhoA is thought to promote the growth of leading processes, while inhibiting Rac1 and Cdc42 can prevent its growth. At the tip of the leading process, the positive end of the microtubule binds to actin, forming a terminal web. While in the middle of the leading process, stathmin is a protein that takes the role of microtubule destabilizer. In addition, γ-tubulin, along with microtubule protein ninein, are vital for microtubule reconstruction, and exist extensively in the neurons [30]. Second, Cdc42 prevails primarily in the perinuclear region in the forward movement of the centrosome, which involves PARD6α and protein kinase PKCξ; while repositioning of centrioles involves GSK3β, PKCξ, and microfilaments. And again, the nucleus moves toward the centriole, and nuclear movement attributes to the participation of microtubule dynamic complex of dynein. Proteins interacting with the complex encompass dynactin, Ndel1, Lis1, DISC1, and DCX (doublecortin). DCX binds to the microtubules connecting the centriole to the nucleus, an event which may involve Ca2 + signaling [31, 32]. Different proteins of the KASH domain anchor the nucleus to the centriole and the cell membrane. The neurofilament may contribute to the binding of the nucleus to the cell cortex. Finally, the trailing process undergoes retraction, which is an event left to be explored in-depth, although PTEN signaling and actomyosin at the cell ends may play a great part.
Modes of Neuroblast Migration
Two modes of neuroblast migration have been known based on the direction of migration: tangential and radial migration. The former one indicates that cells migrate in a direction parallel to the pial surface and travel to an appropriate site over a long distance, while the latter one refers to that cells follow a trajectory that is perpendicular to the neuroepithelial surface where the neurons migrate from inside out to a specific site to develop cortex. Regarding medium of migration, neuroblast migration is classified into gliophilic migration, neurophilic migration, and vasophilic migration [33,34,35].
Gliophilic Migration (Migration Along Radial Glia Cell)
Gliophilic migration is defined as migration of cells using long radial processes of radial glia as a scaffold [36,37,38]. Rakic et al. [39] proposed this term for the first time and described that in the development of neocortex, neuroblasts migrate along the radial processes vertically. Both in vivo [40, 41] and in vitro [42, 43] experiments showed that radial glial cells are major factors in the migration of neuroblasts. Neuroblasts migrate from the ventricular zone to the cortical plate in the following steps (Fig. 5): (1) Binding to radial glial cells; (2) Migrating as radial glial cells, presenting as reverse movement or lateral movement in the process of forward movement, so called dance sign [42]: neuroblasts do not appear to be tightly adhered to radial glial guide fibers at this stage, and are capable of moving tangentially. Some neuroblasts have been observed to move and return to their original locations. Some neuroblasts have been investigated to extend a process toward the ventricle. (3) Detaching from radial glial cells, and settling in the cortex, differentiating and finally maturing. Neuroblast migration requires complicated intermolecular interactions involving a variety of transmembrane receptors, intracellular signaling molecules, transcription factors (TF), extracellular matrix (ECM), diffusion factors, and adhesion factors [3, 6, 14, 17, 27, 41, 43,44,45,46,47]. Currently, “glial cell-derived signaling” and “complicated intermolecular interactions between long radial processes and neuroblasts” have attracted great attention.
Neuroblasts during migration can generate astrotactin, a glycoprotein that is the first factor shown to mediate neuron-glial interactions. In the cerebellar microcultures experiment, astrotactin involved in the adhesion of neuroblasts to neuron-glial cells increased [48], and mice without the expression of this factor showed decreased glial adhesion and radial migration. Integrin, which mediates intercellular and intercellular-matrix interactions, has also been ascertained to affect neuronal-glial cell adhesion in the radial migration. In mice brains without integrin expression, radial migration was significantly reduced [49] because function-blocking antibodies against integrin induced the detachment of migrating cells and radial glial cells. Real-time imaging reveals that NPCs stretch out small processes to wrap around the radial glial fibers along the migratory direction. As the neurons tightly bind to the glial cells, the cell body moves forward in a jumping manner. Radial glial cells guide neuronal migration, while neurons conversely affect function of radial glial cells. Neuron-glial cell adhesion and neuronal diffusing factors induce the extension of glial cell processes. For instance, migrating neurons in the cortex can generate glial growth factor (GGF), which promotes the maintenance and elongation of radial glial cells [50].
Neurophilic Migration (Migration Along Neuron Chains)
Neurophilic migration is distinct from glial cell migration in the way that migrating cells act as scaffolds for each other and influence migration of each other, as is represented by neuroblast migration from the SVZ to the OB. Meanwhile, upon reaching the OB, these cells will differentiate into interneurons. Numerous signaling molecules are related to this migration pathway. Chain migration is a unique manifestation of cell migration in the RMS, where neuroblasts migrate from the V-SVZ to the OB in a chain-like interlocking arrangement through the connections between them [17, 51] (Fig. 6). Signaling molecules involved in the migration chain of neuroblasts include PSA-NCAM (Polysialic acid-Neural cell adhesion molecule), slit family, integrin family, unknown ASTN-derived factor, cyc-lin-dependent kinase, ErbB4, GABA, and prokineticin 2 receptor (PKR2), some of which join in the connections between neuroblasts, and some control cell motility or cell-ECM interactions [52]. Further studies are needed to clarify the mechanisms of why and when neuroblasts choose such migration pattern.
Vasophilic Migration (Migration Along Blood Vessels)
In vasophilic migration, cells undergoing migration use blood vessels as scaffolds [33]. Experiments show that cerebral neuroblasts in both normal physiological [53] and pathological states [54] may also migrate using blood vessels as scaffolds [33,34,35].
The experimental results of Ono et al. [55] firstly verified that the migration of neuroblasts depends on vascular guidance. The neuroblasts in the OB are closely connected with the vasculature. Once they reach the OB, the neuroblasts separate from the migratory chain and no longer migrate tangentially, initiating radial migration to different sites. Chen Wenjing et al. [56] corroborated blood vessels and radial glial cells remain consistent distribution and direction during cerebellar development and keep a mutual induction relationship, which suggest that blood vessels could guide the migration of neuroblasts at the same time.
Yu Qi et al. [57] demonstrated that cloned neuronal cell lines implanted in the mouse brain also migrate along the vasculature. In addition to OB, neuroblasts migration along the vasculature occur also in RMS. Blood vessels are densely packed in RMS, where the vessels are parallel to the migratory stream and in close proximity to the migrating cells, and the specialized substrates generated by the vessels in the SVZ stop at the ventricular cells, allowing the ventricles to maintain contact with the NSC-rich sites in the SVZ, hence maintaining their microenvironment [58]. Parallel vessels are clustered for reconstruction in the center of the OB where tangential migration is changed to radial migration correspondingly, and blood vessels are clustered and parallel to tangential migration (or radial migration). These findings showed that neuroblasts during migration take the blood vessels as a scaffold and migrate from the SVZ to the granule cell layer and the glomerular layer of the OB. Real-time imaging reveals two complementary modes of neuroblast migration. In the first mode, neuroblasts present linear migration, cell bodies, and anterior processes being close to the blood vessels; in the second mode, only the processes are close to the blood vessels. The probable reason for the second mode is the migrating NPCs encounter some physical constraints (e.g., quiescent state of cell bodies of neuroblasts or astrocytes) when moving along the blood vessels, so neuroblasts migrate by close adhesion to the vessels through the anterior process. The above studies demonstrate that blood vessels play a key role in the migration of neuroblasts.
Regulatory Mechanisms of Vasophilic Migration
Role of Brain-Derived Neurotrophic Factor (BDNF) in Neuroblast Migration with Blood Vessels
RMS, a migratory route that originates in the SVZ of the brain, migrates to reach the OB, where neuroblasts migrate vertically along the scaffold provided by microvascular endothelial cells (MEC) [47]. Within this microvascular network, humeral signals required for the survival and differentiation of neurons become the components of the vascular niche. BDNF secreted from endothelial cells can induce neuroblasts to migrate to the neighboring microvascular territory, working as a neutrophilic factor to maintain neuroblast survival [53]. BDNF fosters neuronal migration via p75NTR (low-affinity BDNF receptor), and neuroblasts in migration can express not only p75NTR but also GABA. Astrocytes neighboring blood vessels stretch out processes to wrap around neuroblasts and express TrkB (high-affinity BDNF receptor). After a while, the migrating cell will enter the astrocyte membranes through TrkB induced by GABA to enter the quiescent state. Grade et al. [59] reported that in ischemic brain injury, BDNF secreted by endothelial cells induces neuroblasts to migrate to the neighboring vascular territory and acts synergistically with astrocytes to promote the migration around the ischemic focus. Wu et al. [1] reported that neuroblasts migrated along the activated astrocytic tunnel, directed by BDNF gradient between subventricular zone (SVZ) and injured cortex after traumatic brain injury. To sum up, the vascular migration of neuroblasts in the adult brain has been found to have a link with the interaction among endothelial cells, astrocytes, and neuroblasts.
Role of Stromal Cell-Derived Factor 1 α(SDF-1α) in Neuroblast Migration with Blood Vessels
Factors that guide and support the vascular migration pathway of neurons also include endothelial and pericyte-derived cytokines, such as stromal cell-derived factor 1α (SDF-1α) in neuroblast migration. CXCR4, the receptor for SDF-1α, is expressed in neurons of developing and mature brain. SDF-1α and CXCR4 are key factors in the neuroblasts migration [60, 61]. Kokovay et al. [62] discovered highly expressed SDF-1α in the capillary beds within the lateral ventricles, which induces CXCR4-expressing neuroblasts to leave the ependymal environment, and upregulate the integrin. Previous experimental results [44, 63] showed that vascular migration and chain migration of neuroblasts in the brain following stroke are dependent on β1 integrin [64]. The daughter cells generated by the transient proliferation of NSCs can express the laminin receptor α6β1 integrin. Shen Q et al. [65] found that NSCs in the SVZ anchor the vascular basement membrane throughα6β1 integrin. If the cell adhesion and signal transduction mobilized by α6β1 are inhibited, the diffusion of NSCs can be facilitated, suggesting that the interactions between NSCs and laminin can inhibit the diffusion of NSCs in the vascular territory and synergistically regulate the migration of neuroblasts along ventricular mircrovasculature. Zhang et al. [66] in their experiment showed that SDF-1α/CXCR4 signaling in the embryonic brain can regulate the vascular migration of oligodendrocyte precursor cells (OPCs).
Role of Vascular Endothelial Growth Factor (VEGF) in Neuroblast Migration with Blood Vessels
Vascular endothelial growth factor (VEGF) can directly provide neuroprotection and nutrient supply to nerve cells and glial cells. Both VEGF and VEGFR2 can be expressed in NSC. Administration of VEGF helped establish a vascular niche, reduce infraction rate, and improve neurological recovery after stroke [67]. Moreover, studies on VEGF-overexpressing transgenic mice confirmed neurogenesis is increased in the SVZ, suggesting VEGF could increase the proliferation and survival of the neuroblasts via its VEGFR2 [68]. Furthermore, in vitro experiment demonstrated that VEGF is capable of enhancing neuronal survival as well as inducing axonal growth [69]. Another experiment found VEGF can induce neuroblast migration by releasing chemoattractant through signal pathways [70]. Carmen et al. [71] proved the deposits of matrix-binding VEGF isoforms could guide accurate granule cell migration. All these findings certified the pivotal role of VEGF in neuroblast migration, demonstrating that blood vessels within development stages also regulate the migration through VEGF secretion [45, 72]
Role of Angiopoietin-1 (Ang-1) in Neuroblast Migration with Blood Vessels
Angiopoietin-1(Ang1), secreted from vascular endothelial cells, acted as chemoattractant for neuroblasts, induced neuroblasts to migrate toward the injured area through the cognitive receptors Tie2 [35, 73]. Ang1 expression was observed within blood vessels extending from the infarct core to SVZ along the pathway of neuroblast migration and adjacent to cells positive for Tie2 [35]. Lin et al. [74] confirmed that following stroke induction, Ang-1/Tie2 are distinct in time and distribution. Ang-1 plays a critical role in the late stage of angiogenesis, as well as vascular remodeling and maturation; while Tie2 is distributed almost exclusively in endothelial cells and is essential for vascular remodeling. According to Beck et al. [75], the upregulation of Ang-1/Tie2 following ischemia allows for the maturation of neovasculature, thus ensuring the maintenance of functional cerebral vasculature. As reported in some studies [76], Ang-1 mRNA and Tie2 expression increased several hours into stroke, and peaked on the 3rd post-stroke day, which lasted for 7 days. Moreover, Lin et al. [74] revealed that Ang-1 mRNA was transiently expressed after stroke and highly expressed at 1–2 weeks.
Role of Extracellular Matrix (ECM) Proteins in Neuroblast Migration with Blood Vessels
In addition to the above diffusible factors, molecules regulating neuroblasts migration with blood vessels were recently revealed [47, 64]. Vascular basement membrane contains many extracellular matrix (ECM) proteins, such as type 4 collagen, laminin, and fibronectin, which are produced by endothelial cells, vessel-enwrapping pericytes, and astrocytes [47]. The integrins are transmembrane receptors that mediate cell adhesion to the ECM, which is involved in the migration of various cell types. In the adult V-SVZ, NSCs and their progenies including neuroblasts expressβ1 integrins, which bind to multiple ECM proteins.β1 integrin is required for the vasculature-guided migration of neuroblasts toward a lesion in the post-stroke brain [64]. The laminin-integrin-dependent adhesion of neuroblasts to a scaffolding substrate facilitates their migration in vitro [64].
Taken together, after brain injury, chemoattractive/trophic factors such as BDNF, SDF-1, VEGF and Ang-1, and ECM, these factors triggered the vasculature-guided migration of neuroblasts toward the injury (Table 1): (1) Astrocytes modulate the local concentration of BDNF by capturing it with the high-affinity receptor TrkB, while neuroblasts express a low-affinity BDNF receptor p75NTR. This mechanism regulated vasculature-guided neuroblasts migration. (2) SDF-1 and Ang1, secreted from vascular endothelial cells, acted as chemoattractant for neuroblasts, induced neuroblasts to migrate toward the injured area through the cognitive receptors CXCR4 and Tie2, respectively. In other words, SDF1/CXCR4 and Ang-1/Tie2 signaling regulated the neuroblasts migration along blood vessels. (3) VEGF / VEGFR signaling also assisted the vascular migration of neuroblasts. (4) The interaction of β1 integrin expressed in neuroblasts and laminin, an ECM protein composing the vascular basal lamina, facilitates neuroblast migration using vascular scaffolds. However, the role of other factors in the vasculature-guided neuroblasts migration needs to be further explored.
Neuroblast Migration in TBI and Other Types of Brain Injury
After TBI or other types of brain injury, some of the neuroblasts in the SVZ migrate toward the site of injury to repopulate the injured tissues [1, 26, 77]. The notable migratory capacity of SVZ-derived neuroblasts is essential for efficient neuronal regeneration in remote areas of the brain. As these neuroblasts migrate for long distances through brain tissues, they are supported by various guidance cues BDNF [1, 17, 26, 53, 59, 78, 79], SDF-1α [17, 26, 60, 61, 78, 80], VEGF [17, 71, 78], and Ang-1 [17, 73, 78], as chemoattractants.
Lee et al. [12] described that the neural progenitors in ischemic striatum were significantly increased on day 5 and 7 post-subarachnoid hemorrhage. Grade et al. [59] reported that many neural progenitors migrated from the SVZ into ischemic area at 2 weeks after ischemic stroke. In aspiration lesion model, neuroblast migration started 2 days post-lesion, and this migration appeared to be persistent even 2 months after lesion [77]. Wu et al. [1] found that neuroblasts migration initiated as early as day 1 and finally arrived at injured cortex on day 7 after TBI in a controlled cortical impact (CCI) model. Apparent discrepancy in these previous studies might arise from differences in lesion models. The specific mechanism needs to be further explored.
Discussion
Neuroblasts are mainly distributed in the area close to the V-SVZ [17], suggesting their limited ability to reach the injury site. Besides, only portion of the neuroblasts survive and differentiate into mature neurons. In a rodent stroke model, only about 0.2% of the dead neurons were replaced by these new neurons. Thus, the number of new neurons in affected areas that are remote from the V-SVZ (the lateral striatum and neocortex) should be increased to induce an efficient recovery from neurological dysfunction in various pathologies. In experimental animals, the infusion of growth and neurotrophic factors and paracrine signaling molecules successfully enhanced the number of V-SVZ neuroblasts and new neurons in the injured brain.
Most studies have aimed for proliferation and survival of neuroblasts in clinical applications, while a few have focused on promoting neuroblast migration toward the injury site [17]. Efficiency of growth or neurotrophic factors is enhanced if they are administered with biocompatible hydrogels. Artificial scaffolding can also enhance neuronal migration to an injury. Ajioka et al. demonstrated that in neonatal mice, a laminin-rich porous sponge transplanted into the injured cortex functions as a migration scaffold for V-SVZ–derived neuroblasts, leading to the increased number of neuroblasts that reach the lesion [81]. In adult mice after stroke, injectable hydrogels enriched with laminin induced efficient migration of neuroblasts from the V-SVZ toward the striatum [64]. Microfiber or nanofiber biomaterials also improved the migration of neuroblasts from the V-SVZ [17]. Artificial scaffolds support not only migration, but also the survival and differentiation processes of the new neurons, which consequently may promote endogenous neuronal regeneration.
Conclusion and Outlook
In summary, neuroblast migration presents three modes according to the characteristics of cells that act as scaffolds during the migration process: gliophilic migration, neurophilic migration, and vasophilic migration (Fig. 7). Many signaling molecules, including BDNF, SDF-1, VEGF, Ang-1, and ECM proteins, affect vascular migration, synergistically regulating the migration of neuroblasts to target areas along blood vessels. However, the precise role of blood vessels in the migration of neuroblasts needs to be further explored. Based on the scaffolding function of blood vessels for cell migration, some experiments have confirmed that artificial scaffolding can promote the migration of nerve cells, thereby restoring nerve function after brain injury [46, 82,83,84,85,86]. The in-depth study of neuroblast migration will most probably provide theoretical basis and breakthrough for the clinical treatment of brain injury diseases.
Data Availability
All data generated during this study are included in this article.
References
Wu N, Sun XC, Zhou C, Yan J, Cheng JC (2023) Neuroblasts migration under control of reactive astrocyte-derived BDNF: a promising therapy in late neurogenesis after traumatic brain injury. Stem Cell Res Ther 14:1–14. https://doi.org/10.1186/s13287-022-03232-0
Ghaith HS, Nawar AA, Gabra MD, Abdelrahman ME, Nafady MH, Bahbah EI, Ebada MA, Ashraf GM et al (2022) A literature review of traumatic brain injury biomarkers. Mol Neurobiol 59:4141–4158. https://doi.org/10.1007/s12035-022-02822-6
Bressan C, Saghatelyan A (2020) Intrinsic mechanisms regulating neuronal migration in the postnatal brain. Front Cell Neurosci 14:620379. https://doi.org/10.3389/fncel.2020.620379
Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707–1710
Guerrero-Cazares H, Lavell E, Chen L, Schiapparelli P, Lara-Velazquez M, Capilla-Gonzalez V, Clements AC, Drummond G et al (2017) Brief Report: Robo1 regulates the migration of human subventricular zone neural progenitor cells during development. Stem Cells 35:1860–1865. https://doi.org/10.1002/stem.2628
Obernier K, Alvarez-Buylla A (2019) Neural stem cells: origin, heterogeneity and regulation in the adult mammalian brain. Development 146. https://doi.org/10.1242/dev.156059
Boldrini M, Fulmore CA, Tartt AN, Simeon LR, Pavlova I, Poposka V, Rosoklija GB, Stankov A et al (2018) Human hippocampal neurogenesis persists throughout aging. Cell Stem Cell 22(589–599):e585. https://doi.org/10.1016/j.stem.2018.03.015
Dillen Y, Kemps H, Gervois P, Wolfs E, Bronckaers A (2020) Adult neurogenesis in the subventricular zone and its regulation after ischemic stroke: implications for therapeutic approaches. Transl Stroke Res 11:60–79. https://doi.org/10.1007/s12975-019-00717-8
Pushchina EV, Zharikova EI, Varaksin AA, Prudnikov IM, Tsyvkin VN (2020) Proliferation, adult neuronal stem cells and cells migration in pallium during constitutive neurogenesis and after traumatic injury of telencephalon of juvenile masu salmon, Oncorhynchus masou. Brain Sci 10. https://doi.org/10.3390/brainsci10040222
Buchsbaum IY, Cappello S (2019) Neuronal migration in the CNS during development and disease: insights from in vivo and in vitro models. Development 146. https://doi.org/10.1242/dev.163766
Akter M, Kaneko N, Sawamoto K (2021) Neurogenesis and neuronal migration in the postnatal ventricular-subventricular zone: similarities and dissimilarities between rodents and primates. NEUROSCI RES 167:64–69. https://doi.org/10.1016/j.neures.2020.06.001
Esteve D, Molina-Navarro MM, Giraldo E, Martínez-Varea N, Blanco-Gandia M-C, Rodríguez-Arias M, García-Verdugo JM, Viña J et al (2021) Adult neural stem cell migration is impaired in a mouse model of Alzheimer’s disease. Mol Neurobiol 59:1168–1182. https://doi.org/10.1007/s12035-021-02620-6
Moreno-Jimenez EP, Flor-Garcia M, Terreros-Roncal J, Rabano A, Cafini F, Pallas-Bazarra N, Avila J, Llorens-Martin M (2019) Adult hippocampal neurogenesis is abundant in neurologically healthy subjects and drops sharply in patients with Alzheimer’s disease. Nat Med 25:554–560. https://doi.org/10.1038/s41591-019-0375-9
Zheng W, ZhuGe Q, Zhong M, Chen G, Shao B, Wang H, Mao X, Xie L et al (2013) Neurogenesis in adult human brain after traumatic brain injury. J Neurotrauma 30:1872–1880. https://doi.org/10.1089/neu.2010.1579
Nakajima C, Sawada M, Sawamoto K (2021) Postnatal neuronal migration in health and disease. Curr Opin Neurobiol 66:1–9. https://doi.org/10.1016/j.conb.2020.06.001
Jara N, Cifuentes M, Martinez F, Salazar K, Nualart F (2016) Cytoarchitecture, proliferative activity and neuroblast migration in the subventricular zone and lateral ventricle extension of the adult guinea pig brain. Stem Cells 34:2574–2586. https://doi.org/10.1002/stem.2430
Kaneko N, Sawada M, Sawamoto K (2017) Mechanisms of neuronal migration in the adult brain. J Neurochem 141:835–847. https://doi.org/10.1111/jnc.14002
Marín O, Rubenstein JL (2001) A long, remarkable journey: tangential migration in the telencephalon. Nat Rev Neurosci 2:780–790. https://doi.org/10.1038/35097509
Marin O, Valdeolmillos M, Moya F (2006) Neurons in motion: same principles for different shapes? Trends Neurosci 29:655–661. https://doi.org/10.1016/j.tins.2006.10.001
Vandestadt C, Vanwalleghem GC, Khabooshan MA, Minna-Liisa A ¨nko¨, Scott EK, Kaslin J (2021) RNA-induced inflammation and migration of precursor neurons initiates neuronal circuit regeneration in zebrafish. Dev Cell 56:2364-2380.e2368. https://doi.org/10.1016/j.devcel.2021.07.021
Xiong Y, Zhang Y, Mahmood A, Chopp M (2015) Investigational agents for treatment of traumatic brain injury. Expert Opin Investig Drugs 24:743–760. https://doi.org/10.1517/13543784.2015.1021919
Quillinan N, Herson PS, Traystman RJ (2016) Neuropathophysiology of brain injury. Anesthesiol Clin 34:453–464. https://doi.org/10.1016/j.anclin.2016.04.011
Ngwenya LB, Danzer SC (2018) Impact of traumatic brain injury on neurogenesis. Front Neurosci 12:1014. https://doi.org/10.3389/fnins.2018.01014
Lim DA, Alvarez-Buylla A (2016) The adult ventricular-subventricular zone (V-SVZ) and olfactory bulb (OB) neurogenesis. Cold Spring Harb Perspect Biol 8. https://doi.org/10.1101/cshperspect.a018820
Codega P, Silva-Vargas V, Paul A, Maldonado-Soto AR, Deleo AM, Pastrana E, Doetsch F (2014) Prospective identification and purification of quiescent adult neural stem cells from their in vivo niche. Neuron 82:545–559. https://doi.org/10.1016/j.neuron.2014.02.039
Chang EH, Adorjan I, Mundim MV, Sun B, Dizon ML, Szele FG (2016) Traumatic brain injury activation of the adult subventricular zone neurogenic niche. Front Neurosci 10:332. https://doi.org/10.3389/fnins.2016.00332
Minegishi T, Inagaki N (2020) Forces to drive neuronal migration steps. Front Cell Dev Biol 8:863. https://doi.org/10.3389/fcell.2020.00863
Francis F, Cappello S (2021) Neuronal migration and disorders – an update. CURR OPIN NEUROBIOL 66:57–68. https://doi.org/10.1016/j.conb.2020.10.002
Stoufflet J, Chaulet M, Doulazmi M, Fouquet C, Dubacq C, Métin C, Schneider-Maunoury S, Trembleau A et al (2020) Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration. Sci Adv 6:eaba3992. https://doi.org/10.1126/sciadv.aba3992
Theisen U, Ernst AU, Heyne RLS, Ring TP, Thorn-Seshold O, K¨oster RW (2020) Microtubules and motor proteins support zebrafish neuronal migration by directing cargo. J Cell Biol 219. https://doi.org/10.1083/jcb.201908040
Horigane S-I, Ozawa Y, Yamada H, TakemotoKimura S (2019) Calcium signaling: a key regulator of neuronal migration. Am J Hum Genet 105:1126–1147. https://doi.org/10.1093/jb/mvz012/5316037
Nakagawa-Tamagawa N, Kirino E, Sugao K, Nagata H, Tagawa Y (2021) Involvement of calcium-dependent pathway and β subunit-interaction in neuronal migration and callosal projection deficits caused by the Cav1.2 I1166T mutation in developing mouse neocortex. Front Neurosci 15:747951. https://doi.org/10.3389/fnins.2021.747951
Bovetti S, Hsieh YC, Bovolin P, Perroteau I, Kazunori T, Puche AC (2007) Blood vessels form a scaffold for neuroblast migration in the adult olfactory bulb. J Neurosci 27. https://doi.org/10.1523/JNEUROSCI.0678-07.2007
Saghatelyan A (2009) role of blood vessels in the neuronal migration. Semin Cell Dev Biol 20:744–750. https://doi.org/10.1016/j.semcdb.2009.04.006
Marta Segarra CB, Kirchmaier A-P (2015) A vascular perspective on neuronal migration. Mech Dev 138:17–25. https://doi.org/10.1016/j.mod.2015.07.004
Meyerink BL, Tiwari NK, Pilaz LJ (2020) Ariadne's thread in the developing cerebral cortex: mechanisms enabling the guiding role of the radial glia basal process during neuron migration. Cells 10. https://doi.org/10.3390/cells10010003
Sokpor G, Kerimoglu C, Nguyen H, Pham L, Rosenbusch J, Wagener R, Nguyen HP, Fischer A et al (2021) Loss of BAF complex in developing cortex perturbs radial neuronal migration in a WNT signaling-dependent manner. Front Mol Neurosci 14:687581. https://doi.org/10.3389/fnmol.2021.687581
Morozov YM, Mackie K, Rakic P (2020) Cannabinoid type 1 receptor is undetectable in rodent and primate cerebral neural stem cells but participates in radial neuronal migration. Int J Mol Sci 21. https://doi.org/10.3390/ijms21228657
Rakic P (1971) Guidance of neurons migrating to the fetal monkey neocortex. Brain Res 33:471–476. https://doi.org/10.1016/0006-8993(71)90119-3
Anton E, Cameron R, Rakic P (1996) Role of neuron-glial junctional domain proteins in the maintenance and termination of neuronal migration across the embryonic cerebral wall. J Neurosci 16:2283–2293. https://doi.org/10.1083/jcb.106.2.505
Edmondson J, Hatten M (1987) Glial-guided granule neuron migration in vitro_ a high-resolution time-lapse video microscopic study. J Neurosci 7:1928–1934
Kriegstein AR, Noctor SC (2004) Patterns of neuronal migration in the embryonic cortex. Trends Neurosci 27:392–399. https://doi.org/10.1016/j.tins.2004.05.001
Noctor S, Flint A, Weissman T, Kriegstein A (2001) Neurons derived from radial glial cells establish radial units in neocortex. Nature 408:714–720
Sun Z, Guo SS, Fassler R (2016) Integrin-mediated mechanotransduction. J Cell Biol 215:445–456. https://doi.org/10.1083/jcb.201609037
Li S, Kumar TP, Joshee S, Kirschstein T, Subburaju S, Khalili JS, Kloepper J, Du C et al (2018) Endothelial cell-derived GABA signaling modulates neuronal migration and postnatal behavior. Cell Res 28:221–248. https://doi.org/10.1038/cr.2017.135
Jinnou H, Sawada M, Kawase K, Kaneko N, Herranz-Perez V, Miyamoto T, Kawaue T, Miyata T et al (2018) Radial glial fibers promote neuronal migration and functional recovery after neonatal brain injury. Cell Stem Cell 22(128–137):e129. https://doi.org/10.1016/j.stem.2017.11.005
Fujioka T, Kaneko N, Sawamoto K (2019) Blood vessels as a scaffold for neuronal migration. Neurochem Int 126:69–73. https://doi.org/10.1016/j.neuint.2019.03.001
Edmondson J, Liem R, Kuster J, Hatten M (1988) Astrotactin_ a novel neuronal cell surface antigen that mediates neuron- astroglial interactions in cerebellar microcultures. J Cell Biol 106:505–517. https://doi.org/10.1083/jcb.106.2.505
Anton E, Kreidberg J, Rakic P (1999) Distinct functions of alpha3 and alpha integrin receptors in neuronal migration and laminar organization of the cerebral cortex. Neuron 22:277–289. https://doi.org/10.1016/s0896-6273(00)81089-2
Rio C, Rieff H, Qi P, Corfas G (1997) Neuregulin and erbB receptors play a critical role in neuronal migration. Neuron 19:39–50. https://doi.org/10.1016/s0896-6273(00)80346-3
Peretto P, Giachino C, Aimar P, Fasolo A, Bonfanti L (2005) Chain formation and glial tube assembly in the shift from neonatal to adult subventricular zone of the rodent forebrain. J Comp Neurol 487:407–427. https://doi.org/10.1002/cne.20576
Yamagishi S, Bando Y, Sato K (2021) Involvement of netrins and their receptors in neuronal migration in the cerebral cortex. Front Cell Dev Biol 8:590009. https://doi.org/10.3389/fcell.2020.590009
Snapyan M, Lemasson M, Brill MS, Blais M, Massouh M, Ninkovic J, Gravel C, Berthod F et al (2009) Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling. J Neurosci 29:4172–4188. https://doi.org/10.1523/jneurosci.4956-08.2009
Ohab JJ, Carmichael ST (2008) Poststroke neurogenesis: emerging principles of migration and localization of immature neurons. Neuroscientist 14:369–380. https://doi.org/10.1177/1073858407309545
Ono K, Tomasiewicz H, Magnuson T, Rutishauser U (1994) N-CAM mutation inhibits tangential neuronal migration and is phenocopied by enzymatic removal of polysialic acid. Neuron 13:595–609. https://doi.org/10.1016/0896-6273(94)90028-0
Chen WJ, Zhang WL, Li W, Wang Q, Liu B, Deng JB (2013) Interaction between cell migration and Vasculature in the deVeloping cerebeUum of the mouse. Acta Anatomica Sinica 44:740–747. https://doi.org/10.3969/j.issn.0529—1356.2013.06.003
Yu Q, Zuou QS, Zhao X, Liu QX (2012) Current progress in functions of axon guidance molecule Slit and underlying molecular mechanism. Acta Physiologica Sinica 64:220–230
Doetsch F (2003) A niche for adult neural stem cells. Curr Opin Genet Dev 13:543–550. https://doi.org/10.1016/j.gde.2003.08.012
Grade S, Weng YC, Snapyan M, Kriz J, Malva JO, Saghatelyan A (2013) Brain-derived neurotrophic factor promotes vasculature-associated migration of neuronal precursors toward the ischemic striatum. PLoS ONE 8. https://doi.org/10.1371/journal.pone.0055039
Wang RY, Yang YR, Chang HC (2022) The SDF1-CXCR4 axis is involved in the hyperbaric oxygen therapy-mediated neuronal cells migration in transient brain ischemic rats. Int J Mol Sci 23. https://doi.org/10.3390/ijms23031780
Ma S, Zhou J, Huang T, Zhang Z, Xing Q, Zhou X, Zhang K, Yao M et al (2021) Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury. Acta Biomater 131:185–197. https://doi.org/10.1016/j.actbio.2021.06.038
Kokovay E, Goderie S, Wang Y, Lotz S, Lin G, Sun Y, Roysam B, Shen Q et al (2010) Adult SVZ lineage cells home to and leave the vascular niche via differential responses to SDF1/CXCR4 signaling. Cell Stem Cell 7:163–173. https://doi.org/10.1016/j.stem.2010.05.019
Paul NR, Jacquemet G, Caswell PT (2015) Endocytic trafficking of integrins in cell migration. Curr Biol 25:R1092-1105. https://doi.org/10.1016/j.cub.2015.09.049
Fujioka T, Kaneko N, Ajioka I, Nakaguchi K, Omata T, Ohba H, Fassler R, Garcia-Verdugo JM et al (2017) beta1 integrin signaling promotes neuronal migration along vascular scaffolds in the post-stroke brain. EBioMedicine 16:195–203. https://doi.org/10.1016/j.ebiom.2017.01.005
Shen Q, Wang Y, Kokovay E, Lin G, Chuang SM, Goderie SK, Roysam B, Temple S (2008) Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions. Cell Stem Cell 3:289–300. https://doi.org/10.1016/j.stem.2008.07.026
Tsai H, Niu J, Munji R, Fancy S (2016) Oligodendrocyte precursors migrate along vasculature in the developing nervous system. Science 351:379–384. https://doi.org/10.1126/science.aad3839
Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg DA (2003) VEGF-induced neuroprotection, neurogenesis, and angiogenesis after focal cerebral ischemia. J Clin Investig 111:1843–1851. https://doi.org/10.1172/jci200317977
Wang Y, Jin K, Mao XO, Xie L, Banwait S, Marti HH, Greenberg DA (2007) VEGF-overexpressing transgenic mice show enhanced post-ischemic neurogenesis and neuromigration. J Neurosci Res 85:740–747. https://doi.org/10.1002/jnr.21169
Bagnard D, Vaillant C, Khuth S-T, Dufay N, Lohrum M, Püschel AW, Belin M-F, Bolz J et al (2001) Semaphorin 3A–vascular endothelial growth factor-165 balance mediates migration and apoptosis of neural progenitor cells by the recruitment of shared receptor. J Neurosci 21:3332–3341
Li G, Adesnik H, Li J, Long J, Nicoll RA, Rubenstein JL, Pleasure SJ (2008) Regional distribution of cortical interneurons and development of inhibitory tone are regulated by Cxcl12/Cxcr4 signaling. J Neurosci 28:1085–1098. https://doi.org/10.1523/JNEUROSCI.4602-07.2008
Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D et al (2010) Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1. J Neurosci 30:15052–15066. https://doi.org/10.1523/JNEUROSCI.0477-10.2010
Barber M, Andrews WD, Memi F, Gardener P, Ciantar D, Tata M, Ruhrberg C, Parnavelas JG (2018) Vascular-derived Vegfa promotes cortical interneuron migration and proximity to the vasculature in the developing forebrain. Cereb Cortex 28:2577–2593. https://doi.org/10.1093/cercor/bhy082
Ohab JJ, Fleming S, Blesch A, Carmichael ST (2006) A neurovascular niche for neurogenesis after stroke. J Neurosci 26:13007–13016. https://doi.org/10.1523/jneurosci.4323-06.2006
Lin T-N, Wang C-K, Cheung W-M, Hsu C-Y (2000) Induction of angiopoietin and tie receptor mRNA expression after cerebral ischemia-reperfusion. J Cereb Blood Flow Metab 20:387–395. https://doi.org/10.1097/00004647-200002000-00021
Beck H, Acker T, Wiessner C, Allegrini PR, Plate KH (2000) Expression of angiopoietin-1, angiopoietin-2, and tie receptors after middle cerebral artery occlusion in the rat. Am J Pathol 157:1473–1483. https://doi.org/10.1016/s0002-9440(10)64786-4
Lin T-N, Nian G-M, Chen S-F, Cheung W-M, Chang C, Lin W-C, Hsu CY (2001) Induction of tie-1 and tie-2 receptor protein expression after__cerebral ischemia–reperfusion. J Cereb Blood Flow Metab 21:690–701. https://doi.org/10.1097/00004647-200106000-00007
Saha B, Peron S, Murray K, Jaber M, Gaillard A (2013) Cortical lesion stimulates adult subventricular zone neural progenitor cell proliferation and migration to the site of injury. Stem Cell Research 11:965–977. https://doi.org/10.1016/j.scr.2013.06.006
Sawada M, Matsumoto M, Sawamoto K (2014) Vascular regulation of adult neurogenesis under physiological and pathological conditions. Front Neurosci 8. https://doi.org/10.3389/fnins.2014.00053
Carabalona A, Hu DJ-K, Vallee RB (2016) KIF1A inhibition immortalizes brain stem cells but blocks BDNF-mediated neuronal migration. Nat Neurosci 19:253–262. https://doi.org/10.1038/nn.4213
Li Y, Chang S, Li W, Tang G, Ma Y, Liu Y, Yuan F, Zhang Z et al (2018) cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice. Stem Cell Res Ther 9. https://doi.org/10.1186/s13287-018-0865-6
Ajioka I, Jinnou H, Okada K, Sawada M, Saitoh S, Sawamoto K (2015) Enhancement of neuroblast migration into the injured cerebral cortex using laminin-containing porous sponge. Tissue Eng Part A 21:193–201. https://doi.org/10.1089/ten.tea.2014.0080
Kaneko N, Herranz-Pérez V, Otsuka T, Sano H, Ohno N, Omata T, Nguyen HB, Thai TQ et al (2018) New neurons use Slit-Robo signaling to migrate__through the glial meshwork and approach__a lesion for functional regeneration. Sci Adv 4:eaav0618. https://doi.org/10.1126/sciadv.aav0618
Nih LR, Gojgini S, Carmichael ST, Segura T (2018) Dual-function injectable angiogenic biomaterial for the repair of brain tissue following stroke. Nat Mater 17:642–651. https://doi.org/10.1038/s41563-018-0083-8
Tseng KY, Anttila JE, Khodosevich K, Tuominen RK, Lindahl M, Domanskyi A, Airavaara M (2018) MANF promotes differentiation and migration of neural progenitor cells with potential neural regenerative effects in stroke. Mol Ther 26:238–255. https://doi.org/10.1016/j.ymthe.2017.09.019
Bao M, Xie J, Huck WTS (2018) Recent advances in engineering the stem cell microniche in 3D. Adv Sci (Weinh) 5:1800448. https://doi.org/10.1002/advs.201800448
Muncie JM, Weaver VM (2018) The physical and biochemical properties of the extracellular matrix regulate cell fate. Curr Top Dev Biol 130:1–37. https://doi.org/10.1016/bs.ctdb.2018.02.002
Acknowledgements
We thank Professor Yan Wang (Chongqing Medical University, China) for proofreading this paper.
Funding
This work was supported by the Medical Key Discipline of Chongqing, China, No. zdxk201716; this work was supported by the National Natural Science Foundation of China, No. 82172193 (to C.J.C). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing, or deciding to submit this paper for publication.
Author information
Authors and Affiliations
Contributions
NW designed and wrote the manuscript; NW, WLL, QC, MC, and SYC reviewed the previous studies; WLL drew the figures; CJC and YMX supervised and modified the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics Approval
Not applicable.
Consent to Participate
Not applicable.
Consent for Publication
All authors have seen and approved the manuscript and contributed significantly to this work.
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Highlights
1. The in-depth study of neuroblast migration will most probably provide theoretical basis and breakthrough for the clinical treatment of brain injury diseases.
Na Wu and Wenlang Li contributed equally to this work and share the first authorship.
Chongjie Cheng and Yimin Xie contributed equally to this work and share the corresponding authorship.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Wu, N., Li, W., Chen, Q. et al. Research Advances in Neuroblast Migration in Traumatic Brain Injury. Mol Neurobiol 61, 1–13 (2024). https://doi.org/10.1007/s12035-024-04117-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12035-024-04117-4