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DNA in Squid Synaptosomes

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Abstract

The synthesis of brain metabolic DNA (BMD) is modulated by learning and circadian oscillations and is not involved in cell division or DNA repair. Data from rats have highlighted its prevalent association with the mitochondrial fraction and its lack of identity with mtDNA. These features suggested that BMD could be localized in synaptosomes that are the major contaminants of brain mitochondrial fractions. The hypothesis has been examined by immunochemical analyses of the large synaptosomes of squid optic lobes that are readily prepared and identified. Optic lobe slices were incubated with 5-bromo-2-deoxyuridine (BrdU) and the isolated synaptosomal fraction was exposed to the green fluorescent anti-BrdU antibody. This procedure revealed that newly synthesized BrdU-labeled BMD is present in a significant percent of the large synaptosomes derived from the nerve terminals of retinal photoreceptor neurons and in synaptosomal bodies of smaller size. Synaptosomal BMD synthesis was strongly inhibited by actinomycin D. In addition, treatment of the synaptosomal fraction with Hoechst 33258, a blue fluorescent dye specific for dsDNA, indicated that native DNA was present in all synaptosomes. The possible role of synaptic BMD is briefly discussed.

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Author notes

  1. Carolina Cefaliello

    Present address: Department of Neurology, University of Massachusetts Medical School, Albert Sherman Center 6-1008, 368 Plantation St., Worcester, MA, 01605, USA

Authors and Affiliations

  1. Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy

    Carolina Cefaliello, Marina Prisco, Marianna Crispino & Antonio Giuditta

  2. Accademia di Scienze Fisiche e Matematiche, Via Mezzocannone 8, 80134, Naples, Italy

    Antonio Giuditta

Authors
  1. Carolina Cefaliello
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  2. Marina Prisco
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  3. Marianna Crispino
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  4. Antonio Giuditta
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Correspondence to Antonio Giuditta.

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All authors declare that they have no conflict of interests.

Additional information

Antonio Giuditta is emeritus professor of Physiology

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Cefaliello, C., Prisco, M., Crispino, M. et al. DNA in Squid Synaptosomes. Mol Neurobiol 56, 56–60 (2019). https://doi.org/10.1007/s12035-018-1071-3

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  • DOI: https://doi.org/10.1007/s12035-018-1071-3

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