Skip to main content
SpringerLink
Log in

Influence of mineral phase in mineralization of a biocomposite containing chitosan, demineralized bone matrix and bone ash—in vitro study

  • Published:
Bulletin of Materials Science Aims and scope Submit manuscript

Abstract

A resorbable composite which acts as a active barrier in guided bone regeneration was fabricated using chitosan, demineralized bone matrix and bone ash. Its potential to form bone like apatite in simulated body fluid was assessed in this study. The mechanical strength of these composites was correlated with bone ash ratios and composites with better tensile strength were studied for their acellular bioactivity by incubating in simulated body fluid for 21 days. Composites without bone ash did not show acellular bioactivity which was confirmed by thermogravimetric analysis. In case of biocomposites with bone ash, there was an increase in residual weight indicating the mineralization of the composite. The composite containing bone ash has shown the peaks related to phosphate vibrations in its Fourier-transform infrared spectrum. Scanning micrographs revealed formation of apatite like crystals on its surface. Ca/P ratio was found to be 1·7 which is nearer to that of natural bone. Thus, prepared composites can be used as resorbable biocomposite in maxillofacial and oral defects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmet P, Mithat Y, Erdal C, Sevil S and Cuneyt Tas A 2010 Acta Biomaterialia 6 2282

    Article  Google Scholar 

  • Caplanis N, Sigurdsson T J, Rohrer M D and Wikesjo U M 1997 Int. J. Oral. Maxillofac. Implant 12 634

    Google Scholar 

  • Deepak K, Patnayak P, Divya S, Upadhyay R C, Prasad B T R and Rama Mohan T R 2005 Trends. Biomater. Artif. Organs 18 87

    Google Scholar 

  • Di Martino A, Sittinger M and Risbud M V 2005 Biomaterials 26 5983

    Article  Google Scholar 

  • Toque J A, Herliansyah M K, Hamdi M, Ide-Ektessabi A and Wildan M W 2007 IFMBE Proceedings 15 152

    Article  Google Scholar 

  • Jansen J A, De Ruijter J E, Jansen P T M and Paquay Y G C J 1995 Biomaterials 16 819

    Article  Google Scholar 

  • Jeon O, Song S J, Kang S W, Putnam A J and Kim B S 2007 Biomaterials 28 2763

    Article  Google Scholar 

  • Kikuchi M et al 2004 Biomaterials 25 5979

    Article  Google Scholar 

  • Kokubo T, Kushitani H, Sakka S, Kitsugi T and Yamamuro T 1990 J. Biomed. Mater. Res. 24 721

    Article  Google Scholar 

  • Lee E J, Shin D S, Kim H E, Kim H W, Koh Y H and Jang J H 2009 Biomaterials 30 743

    Article  Google Scholar 

  • Lee E J, Teng S H, Jang T S, Wang P, Yook S W, Kim H E and Koh Y H 2010 Acta Biomaterialia 6 3557

    Article  Google Scholar 

  • Ma L, Gao C, Mao Z, Zhou J, Shen J, Hu X and Han C 2003 Biomaterials 24 4833

    Article  Google Scholar 

  • Mostafa N Y 2005 Mater. Chem. Phys. 94 333

    Article  Google Scholar 

  • Neo M, Nakamura T, Ohtsuki C, Kokubo T and Yamamuro T 1993 J. Biomed. Mater. Res. 27 999

    Article  Google Scholar 

  • Piattelli A, Scarano A, Russo P and Matarasso S 1996 Biomaterials 17 791

    Article  Google Scholar 

  • Qi L X Z, Jiang X, Hu C and Zou X 2004 Carbohydr. Res. 339 2693

    Article  Google Scholar 

  • Schenk R K, Buser D, Hardwick W R and Dahlin C 1994 Int. J. Oral. Maxillofac. Implants 9 13

    Google Scholar 

  • Simon M, Baldoni M, Rossi P and Zaffe D 1994 Int. J. Periodont. Restorative. Dent. 14 166

    Google Scholar 

  • Van Leeuwen A C, Huddleston Slater J J R, Gielkens P F M, De Jong J R, Grijpma D W and Bos R R M 2012 Acta Biomaterialia 8 1422

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bio-Products Laboratory, Central Leather Research Institute, Adyar, Chennai, 600 020, India

    Krithiga Gunasekaran, Santhosh Kumar Baskar, Divya Sapphire Mohan & Thotapalli P Sastry

Authors
  1. Krithiga Gunasekaran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Santhosh Kumar Baskar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Divya Sapphire Mohan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thotapalli P Sastry
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Thotapalli P Sastry.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gunasekaran, K., Baskar, S.K., Mohan, D.S. et al. Influence of mineral phase in mineralization of a biocomposite containing chitosan, demineralized bone matrix and bone ash—in vitro study. Bull Mater Sci 37, 729–733 (2014). https://doi.org/10.1007/s12034-014-0683-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12034-014-0683-0

Keywords

Search

Navigation