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Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens

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Abstract

Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study, Brevibacillus halotolerans 7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5–10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2–12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.

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Data availability

However, for this particular study, we do not have a specific data availability statement to include as the data used are proprietary

Abbreviations

AMPs:

Antimicrobial peptides

AU:

Arbitrary unit

MICs:

Minimum inhibitory concentrations

HPLC:

High-performance liquid chromatography

ISP2:

International streptomyces project-2 medium

KCTC:

Korean collection for type cultures

MRSA:

Methicillin-resistant Staphylococcus aureus

SDS:

Sodium dodecyl sulfate

TSA:

Tryptone soy agar

WHO:

World health organization

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Funding

This work was supported by the University of Science and Technology- the University of Danang (T2023_). This research was also funded by the University of Science and Technology, The University of Danang, under grant number T2023-02-35.

Author information

Authors and Affiliations

  1. The University of Danang, University of Science and Technology, 54 Nguyen Luong Bang St., Danang, 550000, Viet Nam

    Ho Le Han

  2. Faculty of Medicine, Dong A University, Danang, 550000, Viet Nam

    Phu Tran Vinh Pham

  3. Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Song-Gun Kim

  4. University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

    Song-Gun Kim

  5. Institut Sains Biologi, Fakulti Sains, Universiti Malaya, 50603, Kuala Lumpur, Malaysia

    Sook Sin Chan

  6. Department of Chemical Engineering and Material Science, Yuan Ze University, Taoyuan, Taiwan

    Kuan Shiong Khoo

  7. School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore

    Kit Wayne Chew

  8. Department of Chemical Engineering, Khalifa University, Shakhbout Bin Sultan St, Zone 1, Abu Dhabi, United Arab Emirates

    Pau Loke Show

  9. The University of Danang, University of Technology and Education, 48 Cao Thang st, Danang, 550000, Viet Nam

    Thi Ngoc Thu Tran & Phuong Thi Dong Nguyen

  10. Faculty of Food, Dong A University, Danang, 550000, Viet Nam

    Hai Thi Viet Nguyen

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  1. Ho Le Han
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Correspondence to Ho Le Han, Kuan Shiong Khoo or Phuong Thi Dong Nguyen.

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Le Han, H., Pham, P.T.V., Kim, SG. et al. Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00963-0

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