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The Role of Nrf2-ARE Signaling Pathway and Tatarinow Sweetflag Extract to Regulate the Acute Phase of Pilocarpine-Induced Epilepsy in Juvenile Rats

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Abstract

To analyze the role of Nrf2-ARE signaling pathway in the regulation of the acute phase of pilocarpine-induced epilepsy in juvenile rats by Tatarinow Sweetflag Extract (TSE). One hundred and twenty SPF-grade Wistar male rats were were divided into five groups by random number table method, namely, normal group, model group, low-dose TSE group, high-dose TSE group, low-dose TSE + Nrf2 inhibitor Brusatol group (low-dose TSE + BRU group), and high-dose TSE + Nrf2 inhibitor Brusatol group (high-dose TSE + BRU group), with 20 rats in each group. The success rate of modelling in the model group, low-dose TSE group, high-dose TSE group, low-dose TSE + BRU group, high-dose TSE + BRU group were 60.00% (12/20), 65.00% (13/20), 65.00% (13/20), 70.00% (14/20), and 70.00% (14/20), respectively, showing no significant difference (P > 0.05). The latency and incidence of class IV and V, discharge amplitude as well as frequency of rats in the low- and high-dose TSE groups were lower than those in the model group (P < 0.05); the lipid peroxide and malondialdehyde concentrations in hippocampal tissues in the low- and high-dose TSE groups were lower than those in the model group (P < 0.05); The Nrf2, NQO-1 and HO- 1 protein and mRNA expression levels were increased in the low- and high-dose TSE groups compared with the model group (P < 0.05). The therapeutic effect of TSE in rats with acute epilepsy was satisfactory, and its mechanism of action may be related to activation of Nrf2-ARE signaling pathway to reduce the degree of oxidative stress.

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All data generated or used during the study appear in the submitted article.

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Funding

This study was supported by the Guiding project of Liaoning Natural Science Foundation (No. 2019-ZD-0802).

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Authors and Affiliations

  1. Pediatrics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, Liaoning, People’s Republic of China

    Guanghui Jin

  2. School of Nursing, Jinzhou Medical University, Jinzhou, 121000, Liaoning, People’s Republic of China

    Zhuo Wang

  3. Neonatal Intensive Care Unit, The First Affiliated Hospital of Jinzhou Medical University, No. 2, Section 5, Renmin Street, Guta District, Jinzhou, 121000, Liaoning, People’s Republic of China

    Wei Zhou & Guyue Li

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  1. Guanghui Jin
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  2. Zhuo Wang
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  3. Wei Zhou
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  4. Guyue Li
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Contributions

GJ, ZW, WZ, GL designed the study, performed the research and analyzed the data. GL contributed new methods. GJ wrote the paper.

Corresponding author

Correspondence to Guyue Li.

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Every procedure was approved by the Animal Care and Use Committee of the First Affiliated Hospital of Jinzhou Medical University.

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Jin, G., Wang, Z., Zhou, W. et al. The Role of Nrf2-ARE Signaling Pathway and Tatarinow Sweetflag Extract to Regulate the Acute Phase of Pilocarpine-Induced Epilepsy in Juvenile Rats. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00911-y

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  • DOI: https://doi.org/10.1007/s12033-023-00911-y

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