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Composition and Diversity of Endophytic Rhizosphere Microbiota in Apple Tree with Different Ages

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Abstract

In order to determine the underlying mechanism of the senescence occurring in older apple trees, the effects of tree age on the community structure and dominant genus of endophytic rhizosphere bacteria in apple were investigated. The diversity and structure of the bacterial communities and corresponding changes in the dominant genera of endophytic rhizosphere bacteria of apple at different ages (2, 8, 16, 22 years) were compared based on 16S rRNA high-throughput sequencing technology. The results revealed that the longer the tree age, the less the number of ASV in the endophytic bacteria. Moreover, the number of ASV in the endophytic bacteria gradually decreased as the tree age increased, however no significant changes were observed in the alpha diversity. At the phyla level, the relative abundance of Actinobacteria increased, while that of Proteobateria decreased. At the genus level, the relative abundance of Mycobacterium, Chujaibacter, and other genera increased, while the relative abundance of Aquabacterium, Ralstonia, Streptomyces, Asticcacaulis, Hyphomicrobium, Pseudomonas, and Sphingomonas decreased. The reduced relative abundance of endophytic rhizosphere bacteria associated with plant growth and disease resistance may thus be the cause of tree senescence. This work acts as a reference to increases the understanding of plant–microbe interactions.

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Acknowledgements

This research was supported by Hebei Natural Science Foundation (C2021204106; C2022204086), Hebei Province Agricultural Industry System Project (HBCT2023100204, HBCT2021100211), Supported by the earmarked fund for CARS-13, S&T Program of Hebei (20326812D).

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    1. College of Plant Protection, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding, Hebei, China

      Jiyuan Pan, Haibin Wen, Xiaojie Wang, Yanan Liu, Chengmin Shi, Dan Zhao & Xiujun Lu

    2. College of Forestry, Hebei Agricultural University, Baoding, Hebei, China

      Qinglong Dong, Yang Liu & Xuemei Zhang

    3. Hebei Walnut Technical Engineering Research Center, Lincheng, 054300, Hebei, China

      Xuemei Zhang & Xiujun Lu

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    Pan, J., Dong, Q., Wen, H. et al. Composition and Diversity of Endophytic Rhizosphere Microbiota in Apple Tree with Different Ages. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00794-z

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