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Production Enhancement of Bacterial Cellulase Cocktail Using Potato Peels Waste Feedstock and Combination of Water Hyacinth Root and Pea Pod Extract as Natural Nutrient Media: Application in Bioconversion of Potato Peels

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Abstract

Solid wastes are the major contributors in global environmental pollution and their management is the need of urgency towards development of sustainable world. In the present work, solid waste of potato peels has been used as feedstock for fermentation of bacterial cellulase production and substrate for enzymatic hydrolysis via this enzymes cocktail. Additionally, liquid extracts of pea pod and root of water hyacinth wastes have been used to complete nutritional requirements and moisture balance in SSF process during the course of enzyme production. At optimum feedstock concentration of 6.0 g PPW and 10:40 extract-based moisture ratio of WHR and Ppw, Bacillus sp. produced 15 U/gds FP in 18 h, whereas maximum 36 U/gds BGL and 42 U/gds EG have been recorded in 24 h of SSF. Temperature 35 °C and pH 5.5 were optimum for enzyme production while the produced enzyme was thermally stable upto 30 h at 35 °C with 100% pH stability upto 14 h and 77% relative activity at 34 h. The optimized bacterial enzymes have been used for bioconversion of PPW biomass and 26 g/L glucose has been recorded at a hydrolytic temperature of 50 °C and pH 5.0. The study may have feasible promising scope in cellulosic biorefineries and waste management.

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Acknowledgments

All the authors acknowledge their parent institutes. The authors extend their appreciation to the Researchers supporting Project number (RSP2023R56), King Saud University, Riyadh, Saudi Arabia. Author N.S. thankfully acknowledges, DST for WOS-B project (DST/WOS-B/ER-3/2021(G) and Department of Chemical Engineering &Technology, IIT (BHU) Varanasi.

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Authors and Affiliations

  1. Indian Institute of Technology (BHU) Varanasi, Varanasi, 221005, India

    Neha Srivastava, Pathan Ahemad Khan & P. K. Mishra

  2. Department of Chemistry, Institute of Applied Science and Humanities, GLA University, Mathura, 281406, India

    Basant Lal

  3. Department of Environmental Science, Jamia Millia Islamia Central University, New Delhi, 110025, India

    Rajeev Singh

  4. Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia

    Asad Syed & Abdallah M. Elgorban

  5. University Centre for Research & Development, Department of Chemistry, Chandigarh University, Gharuan, Mohali, 140413, India

    Meenakshi Verma

  6. Applied Biology and Biopharmaceuticals Sciences, Department of Science, Atlantic Technological University, Galway, Ireland

    Anthonia O’Donovan

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  1. Neha Srivastava
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  2. Pathan Ahemad Khan
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Correspondence to Neha Srivastava.

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Srivastava, N., Khan, P.A., Lal, B. et al. Production Enhancement of Bacterial Cellulase Cocktail Using Potato Peels Waste Feedstock and Combination of Water Hyacinth Root and Pea Pod Extract as Natural Nutrient Media: Application in Bioconversion of Potato Peels. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00789-w

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