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Hsa_circ_0137652 Regulates miR-1205/CCNB1 Axis to Accelerate the Malignancy of Breast Cancer

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Abstract

CircRNAs have become a hotspot in tumor research owing to their high stability and specific functions. We investigated the function of hsa_circ_0137652 in the onset and progression of breast cancer (BC). The expression of circ_0137652, miR-1205, and CCNB1 in BC tissues and cell lines were detected using RT-qPCR and/or western blotting. Dual-luciferase reporter and RNA immunoprecipitation chip assays were used to confirm any potential connections between circ_0137652, miR-1205, and CCNB1. CCK-8 and clone formation assays (CFA) were used to measure the proliferation of BC cells. The Transwell assay was used to investigate the migration of BC cells, and the impact of circ_0137652 on BC tumor formation in vivo was validated using animal experiments. RT-qPCR results showed that circ_0137652 and CCNB1 in breast cancer tissues were notably upregulated in normal tissues, whereas miR-1205 was prominently downregulated. After silencing circ_0137652, the growth and migration of BC cells were reduced. Animal experiments showed that circ_0137652 hampers the tumorigenesis of BC cells in vivo. Additionally, we found that circ_0137652 functions as a sponge for miR-1205. Moreover, the miR-1205 inhibitor notably facilitated cell proliferation and migration and attenuated the action of circ_0137652 knockdown. Furthermore, miR-1205 inhibits BC progression by targeting CCNB1. Circ_0137652 controls the miR-1205/CCNB1 axis to induce increased breast cancer malignancy. Our findings suggest that circ_0137652 may be a novel target for BC therapy.

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Acknowledgements

None.

Funding

This work was supported by the Youth Project of the Wuhan Municipal Health Commission under the project name “Efficacy and safety of percutaneous acupoint electrical stimulation based on ‘Biaoben acupoints combination’ in the treatment of breast cancer-related fatigue.” (Project Number: S202204110000), [Grant number: WZ22Q18].

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Author notes

  1. Xu Zhou and Qiang Zhao have contributed equally to this work.

Authors and Affiliations

  1. Department of Thyroid and Breast Surgery, Wuhan Red Cross Hospital, No. 392, Hong Kong Road, Jianghan District, Wuhan, 430015, Hubei, China

    Xu Zhou & Min Xiao

  2. Department of Rehabilitation Medicine, Wuhan Hankou Hospital, Wuhan, 430012, Hubei, China

    Qiang Zhao

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Contributions

Both the experiments and the data analysis were done by XZ and QZ. MX conceptualized and created the study. XZ and MX gathered the data. QZ and MX were in charge of data analysis and interpretation. The paper was written by XZ and QZ. The manuscript was reviewed and revised by MX. All authors evaluated and approved the article.

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Correspondence to Min Xiao.

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According to the authors, there were no conflicts of interest.

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This study was authorized by the medical ethics committee of the Wuhan Red Cross Hospital (Wuhan, China). The Declaration of Helsinki's ethical guidelines were followed in the processing of the clinical tissue samples. A form for informed consent was filled out by each patient. All of the patients completed an informed consent form. The animal experiment conducted observed the ARRIVE guidelines and was authorized by the Animal Care and Use Committee of Wuhan Red Cross Hospital (Wuhan, China).

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Zhou, X., Zhao, Q. & Xiao, M. Hsa_circ_0137652 Regulates miR-1205/CCNB1 Axis to Accelerate the Malignancy of Breast Cancer. Mol Biotechnol 65, 1824–1835 (2023). https://doi.org/10.1007/s12033-023-00684-4

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