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Loss-of-Function Mutation of ACTIN-RELATED PROTEIN 6 (ARP6) Impairs Root Growth in Response to Salinity Stress

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Abstract

H2A.Z-containing nucleosomes have been found to function in various developmental programs in Arabidopsis (e.g., floral transition, warm ambient temperature, and drought stress responses). The SWI2/SNF2-Related 1 Chromatin Remodeling (SWR1) complex is known to control the deposition of H2A.Z, and it has been unraveled that ACTIN-RELATED PROTEIN 6 (ARP6) is one component of this SWR1 complex. Previous studies showed that the arp6 mutant exhibited some distinguished phenotypes such as early flowering, leaf serration, elongated hypocotyl, and reduced seed germination rate in response to osmotic stress. In this study, we aimed to investigate the changes of arp6 mutant when the plants were grown in salt stress condition. The phenotypic observation showed that the arp6 mutant was more sensitive to salt stress than the wild type. Upon salt stress condition, this mutant exhibited attenuated root phenotypes such as shorter primary root length and fewer lateral root numbers. The transcript levels of stress-responsive genes, ABA INSENSITIVE 1 (ABI1) and ABI2, were found to be impaired in the arp6 mutant in comparison with wild-type plants in response to salt stress. In addition, a meta-analysis of published data indicated a number of genes involved in auxin response were induced in arp6 mutant grown in non-stress condition. These imply that the loss of H2A.Z balance (in arp6 mutant) may lead to change stress and auxin responses resulting in alternative root morphogenesis upon both normal and salinity stress conditions.

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Data Availability

The data published by Sura et al. [25] were obtained and used for gene ontology (GO) analysis and are available at https://doi.org/10.1105/tpc.16.00573.

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Acknowledgements

We would like to thank Prof. Ilha Lee (Seoul National University, South Korea) for his kindness in providing the arp6 mutant. We sincerely appreciate Dr. Nguyen Minh Tan (UCLA, USA) for his critical reading and comments on the manuscript.

Funding

This work was supported by Ho Chi Minh City Open University.

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Authors and Affiliations

  1. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Bich Hang Do

  2. National Institute of Medicinal Materials, Hanoi, Vietnam

    Nguyen Tuan Hiep

  3. Faculty of Biotechnology, Ho Chi Minh City Open University, 97 Vo Van Tan Street, District 3, Ho Chi Minh, Vietnam

    Thuan Duc Lao & Nguyen Hoai Nguyen

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  1. Bich Hang Do
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  2. Nguyen Tuan Hiep
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Contributions

NHN designed and supervised the research. BHD and NHN performed the experiments. BHD and NHN wrote the manuscript. NTH and TDL read and commented on the manuscript. All authors gave final approval for publication and agree to be held accountable for the work performed therein.

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Correspondence to Nguyen Hoai Nguyen.

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This study was performed on the model plant (Arabidopsis thaliana) and was not involved in human participants and/or animals.

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Do, B.H., Hiep, N.T., Lao, T.D. et al. Loss-of-Function Mutation of ACTIN-RELATED PROTEIN 6 (ARP6) Impairs Root Growth in Response to Salinity Stress. Mol Biotechnol 65, 1414–1420 (2023). https://doi.org/10.1007/s12033-023-00653-x

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