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Insights into the Synergistic Biodegradation of Waste Papers Using a Combination of Thermostable Endoglucanase and Cellobiohydrolase from Chaetomium thermophilum

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Abstract

Enzymatic hydrolysis is considered an efficient and environmental strategy for the degradation of organic waste materials. Compared to mesophilic cellulases, thermostable cellulases with considerable activity are more advantageous in waste paper hydrolysis, particularly in terms of their participation in synergistic action. In this study, the synergistic effect of two different types of thermostable Chaetomium thermophilum cellulases, the endoglucanase CTendo45 and the cellobiohydrolase CtCel6, on five common kinds of waste papers was investigated. CtCel6 significantly enhanced the bioconversion process, and CTendo45 synergistically increased the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 was 5:3. The synergistic degradation products of each paper material were also determined. Additionally, the activities of CTendo45 and CtCel6 were found to be insensitive to various metals at 2 mM and 10 mM ion concentrations. This study gives an initial insight into a satisfactory synergistic effect of C. thermophilum thermostable cellulases for the hydrolysis of different paper materials, which provides a potential combination of enzymes for industrial applications, including environmentally friendly waste management and cellulosic ethanol production.

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Acknowledgements

Authors thank Prof. Duochuan Li from Shandong Agricultural University for technical assistance. This research was supported by National Key Technology R&D Program of China (Grant No. 2015BAD15B05) and Funds of Shandong Double Tops Program (Grant No. SYL2017XTTD11).

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Authors and Affiliations

  1. Department of Mycology, Shandong Agricultural University, Taian, 271018, Shandong, China

    Weiguang Li, Peng Ji & Chao Han

  2. College of Resources and Environment, Shandong Agricultural University, Taian, 271018, Shandong, China

    Qinzheng Zhou, Chengyao Hua & Chao Han

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  1. Weiguang Li
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  2. Peng Ji
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  3. Qinzheng Zhou
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  4. Chengyao Hua
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Correspondence to Chao Han.

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Li, W., Ji, P., Zhou, Q. et al. Insights into the Synergistic Biodegradation of Waste Papers Using a Combination of Thermostable Endoglucanase and Cellobiohydrolase from Chaetomium thermophilum . Mol Biotechnol 60, 49–54 (2018). https://doi.org/10.1007/s12033-017-0043-6

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