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In vitro anticancer activity of Hirudinaria manillensis methanolic extract and its validation using in silico molecular docking approach

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Abstract

Cancer has emerged as a potentially lethal illness, which recently upsurged in the mortality rate. Animal-derived compounds could be promising targets with higher efficacy and low toxicity in anticancer therapy. The present study aimed to explore the presence of anticancer potential compounds in Hirudinaria manillensis methanolic extract and their anticancer potential against various cancer cell types and target identification by Auto dock method. Initially, the identification of bioactive compounds was achieved by GC–MS analysis followed by the anticancer activity by MTT assay against A549, HeLa, MDA-MB-231, MG-63, and MOLT-4. Further, the effect of a lead compound on the cancer cell target was analyzed by the Auto dock method. GC–MS analysis results revealed the presence of 25 different bioactive compounds including anticancer potential compounds, such as Lupeol, Carvacrol, and Demecolcine. Interestingly, MTT assay results demonstrated the anticancer potential of Hirudinaria manillensis extract (LE) against various cancer cell lines, such as A549 (54.60 µg/ml), HeLa (19.93 µg/ml), MDA-MB-231 (20.23 µg/ml), MG-63 (20.04 µg/ml), and MOLT-4 (171.8 µg/ml), respectively. Among these cell types, the maximum inhibition was observed against HeLa with the IC50 concentration of 19.93 µg/ml. Furthermore, Demecolcine compound was docked with the EGFR tyrosine kinase showed the binding affinity of the docked complex was predicted to be − 6.2 kcal/mol. Thus, we conclude that H. manillensis has a significant anticancer effect on human cancer cell lines and could be used as a natural target which paves the way for further studies on biomedical applications in cancer therapeutics.

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Data availability

The corresponding authors are pleased to provide the original data to those who are interested.

Abbreviations

MTT:

3-[4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide

DMEM:

Dulbecco’s Modified Eagle Medium

FBS:

Fetal bovine serum

EGFR:

Epidermal growth factor receptor

GC–MS:

Gas chromatography–mass spectrometry

PDB:

Protein data bank

DMSO:

Dimethyl sulfoxide

MOLT-4:

Human T-Lymphoblast cell line

HeLa:

Human cervical carcinoma cell line

MDA-MB-231:

Human Breast carcinoma cell line

A549:

Human Lung carcinoma cell line

MG-63:

Human Osteosarcoma cell line

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Acknowledgements

The authors are thankful to Trichy Research Institute of Biotechnology PVT for providing the research facilities and technical support to carrying out the research work. We would like to express my sincere gratitude to Dr. E. Angel Jemima for her contributions, continuous support, insightful comments, and suggestions to complete the study.

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Authors and Affiliations

  1. PG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620017, India

    P. Zeebul Trinita Shannan & Susan G. Suganya

  2. Department of Biotechnology, Trichy Research Institute of Biotechnology, Tiruchirappalli, India

    E. Angel Jemima

  3. Department of Zoology, Thanthai Periyar Government Arts and Science College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620017, India

    M. Ramesh

Authors
  1. P. Zeebul Trinita Shannan
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  2. Susan G. Suganya
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  3. E. Angel Jemima
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  4. M. Ramesh
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Contributions

E. Angel Jemima designed the study. All the authors contributed to the current work. All authors have approved the submitted paper.

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Correspondence to Susan G. Suganya.

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Zeebul Trinita Shannan, P., Suganya, S.G., Angel Jemima, E. et al. In vitro anticancer activity of Hirudinaria manillensis methanolic extract and its validation using in silico molecular docking approach. Med Oncol 41, 88 (2024). https://doi.org/10.1007/s12032-024-02321-9

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  • DOI: https://doi.org/10.1007/s12032-024-02321-9

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