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The effect of proinflammatory cytokines on IL-17RA expression in NSCLC

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Abstract

Interleukin-17 receptor (IL-17RA) is essential for proinflammatory cytokine IL-17-mediated pathogenesis of various tumors. IL-17RA is upregulated by some proinflammatory cytokines such as IL-21 and IL-15 and downregulated by IL-2, while the effect of IL-1β, IL-6, IL-8, TNF-α on IL-17RA expression in non-small cell lung caner (NSCLC) remains unknown. Our findings revealed that IL-17RA mRNA was increased in NSCLC tissues compared with the corresponding peritumor tissues (P = 0.0039) and high expression of IL-17RA protein in human NSCLC tissues was significantly associated with histological subtype, primary tumor size and clinical stages (P = 0.033, 0.033 and 0.011, respectively). IL-17RA mRNA expression was positively related to IL-1β, IL-6, IL-8, TNF-α mRNA expression (P = 0.013, 0.0001, 0.002 and 0.010 respectively) in NSCLC tissues. Furthermore, IL-1β, IL-6, IL-8, TNF-α upregulated IL-17RA mRNA and protein in A549 and H460 cells (all P < 0.05). It is suggested that IL-1β, IL-6, IL-8, TNF-α promoted IL-17RA expression in NSCLC and they may involve in IL-17RA signaling in NSCLC.

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Abbreviations

IL-17RA:

Interleukin 17 receptor A

NSCLC:

Non-small cell lung caner

IL-1β:

Interleukin 1β

IL-6:

Interleukin 6

IL-8:

Interleukin 8

TNF-α:

Tumor necrosis factor α

IHC:

Immunohistochemical

qRT-PCR:

Quantitive reverse transcription—polymerase chain reaction

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81072400), the Research Fund for the Doctoral Program of Higher Education of China, the Scientific Research Foundation of Hubei Health Department (No. JX5B54), the Natural Science Foundation of Hubei province (No. 2009CDB148), the Wuhan Planning Project of Science and Technology (No. 201161038340-01) and the Independent Innovation Research Foundation of Huazhong University of Science and Technology (No. 2011JC016).

Conflict of interest

All the authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei, People’s Republic of China

    Qi Huang, Jiao Du, Jinshuo Fan, Zhilei Lv, Xin Qian, Xiuxiu Zhang, Jieli Han, Caiyun Chen, Feng Wu & Yang Jin

  2. Zhongshan Hospital, Xiamen University, 201-209 Hubin Road, Xiamen, 361004, Fujian, People’s Republic of China

    Jiao Du

  3. Department of Respiratory Medicine, Taihe Hospital, Hubei University of Medicine, No. 32, South Renmin Road, Shiyan, 442000, Hubei, People’s Republic of China

    Xin Qian

  4. Department of Respiratory, The First Hospital of Xi’an City, Xi’an, 710002, Shanxi, People’s Republic of China

    Caiyun Chen

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  1. Qi Huang
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  2. Jiao Du
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  10. Yang Jin
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Correspondence to Yang Jin.

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Qi Huang and Jiao Du have contributed equally to this work.

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Huang, Q., Du, J., Fan, J. et al. The effect of proinflammatory cytokines on IL-17RA expression in NSCLC. Med Oncol 31, 144 (2014). https://doi.org/10.1007/s12032-014-0144-z

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  • DOI: https://doi.org/10.1007/s12032-014-0144-z

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