Abstract
Trigeminal neuralgia (TN) brings a huge burden to patients, without long-term effective treatment. This study aimed to explore the differentially expressed genes (DEGs) and related enrichment pathways in patients with TN. This was a study of transcriptome sequencing and bioinformatics analysis of human samples. Whole blood samples were collected from the TN patients and pain-free controls. RNA was extracted to conduct the RNA-sequencing and the subsequent bioinformatics analysis. DEGs between the two groups were derived. Kyoto encyclopedia of genes and genomes (KEGG) and Gene ontology (GO) was used to find the enrichment pathways of DEGs. Protein protein interaction (PPI) network was used to depict the interaction between DEGs and find the most important gene, hub gene. Compared with the control group, there were 117 up-regulated DEGs and 103 down-regulated DEGs in the whole blood of patients in the TN group. Pathway enrichment analysis showed that DEGs were mainly enriched in the neuroimmune and metabolic pathways. The PPI network demonstrated that colony stimulating factor 2 (CSF2) was the most important hub gene in the whole blood of TN patients. This study shows the expression of the transcriptome in the whole blood samples of TN patients. The neuroimmune responses and key hub gene CSF2 in the whole blood cells play a vital role in the occurrence of TN. Our research provides a theoretical basis for the diagnosis and treatments of TN. This study was registered at clinicaltrials.gov in June 2021 (No. NCT04923399).
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Data Availability
The original contributions presented in the study are publicly available. This data can be found here: [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186505].
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Funding
This study was supported by the National Key Research and Development Program of China (No.2018YFC2001905).
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Y.F and C.X conceived the project. T.L and J.G designed the study. T.L, C.X, J.G, Z.H and Y.Z collected the participants. C.X and J.G analyzed the RNA-seq data. T.L, C.X and Y.F wrote the manuscript. All authors read and approved the final manuscript.
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This study was approved by the Ethics Committee of Peking University People’s Hospital (PHB265-01). This study was conducted in accordance with the code of the world Medical Association (Declaration of Helsinki).
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Liu, T., Xu, C., Guo, J. et al. Whole Blood Transcriptome Analysis in Patients with Trigeminal Neuralgia: a Prospective Clinical Study. J Mol Neurosci 74, 16 (2024). https://doi.org/10.1007/s12031-024-02195-6
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DOI: https://doi.org/10.1007/s12031-024-02195-6