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Association of IL-23R and IL-10 variations with Behçet disease: a genetic analysis study

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Abstract

Behçet disease (BD) is an autoimmune and autoinflammatory disease mainly affecting the Silk Road countries. The interindividual severity of BD depends on differences in the polymorphic profiles of the patients. One of the most prominent markers, HLA-B51 positivity, is also observed in 40–60% of patients with BD on the Silk Road. Inflammatory markers such as interleukin 10 (IL-10) and interleukin 23 receptor (IL-23R) are also widely associated with BD etiology. The polymorphisms on these genes may change the susceptibility to BD. In this case-control study, we assessed the associations of IL-10 rs3024498 and IL-23R rs10889677 single-nucleotide polymorphisms (SNPs) with BD susceptibility, if any. Two hundred eighty HLA-B51-positive patients with BD and 300 healthy controls were genotyped for these SNPs using RFLP-PCR. The chi-square test was used for genotyping. We found that IL-23R rs10889677 CC and IL-10 rs3024498 CT genotype frequencies were higher in the BD group than in the control group (p < 0.0001 and p = 0.0293, respectively). The recessive model (AA + CC vs. AC) and combined genotype (AC + CT) results were also statistically significant (p < 0.0001 and p = 0.0364, respectively). We conclude that IL-23R rs10889677 and IL-10 rs3024498 SNPs may be associated with the susceptibility to BD.

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Author information

Authors and Affiliations

  1. Department of Medical Biology, Medical School, Biruni University, 10. Yıl Caddesi Protokol Yolu No: 45 34010 Topkapı, Istanbul, Turkey

    Guven Yenmis

  2. Department of Medical Biology, Cerrahpasa Medical School, Istanbul University, Cerrahpasa, Istanbul, Turkey

    Sema Sabancelebi

  3. Department of Bioinformatics and System Biology, Institute of Natural and Applied Sciences, Gebze Technical University, Kocaeli, Turkey

    Evren Atak

  4. Department of Molecular Biology and Genetics, Biruni University, Istanbul, Turkey

    Sila Basak Yalinkilic

  5. Department of Medical Biology and Genetics, Medical School, Istanbul Aydin University, Istanbul, Turkey

    Tugba Soydas

  6. Department of Family Medicine, Medical School, Yeditepe University, Istanbul, Turkey

    Tumay Sadikoglu

  7. Department of Family Medicine, Biruni University Hospital, Istanbul, Turkey

    Tumay Sadikoglu

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  1. Guven Yenmis
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Contributions

GY designed and supervised the study; TSa provided the study samples; SS, SBY, and EA performed the experiments; GY, TSo, and TSa performed the data analysis and wrote the paper. All the authors have accepted their responsibility for the entire content of this manuscript and have approved its submission.

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Correspondence to Guven Yenmis.

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All authors stated that the protocol for the research project had been approved by the Institutional Ethical Committee of Biruni University (Decision no:2021/55-16).

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Yenmis, G., Sabancelebi, S., Atak, E. et al. Association of IL-23R and IL-10 variations with Behçet disease: a genetic analysis study. Immunol Res 72, 293–298 (2024). https://doi.org/10.1007/s12026-023-09433-w

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  • DOI: https://doi.org/10.1007/s12026-023-09433-w

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