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Expression of human REG family genes in inflammatory bowel disease and their molecular mechanism

  • REG genes and IBD
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Abstract

The pathophysiology of inflammatory bowel disease (IBD) reflects a balance between mucosal injury and reparative mechanisms. Some regenerating gene (Reg) family members have been reported to be expressed in Crohn’s disease (CD) and ulcerative colitis (UC) and to be involved as proliferative mucosal factors in IBD. However, expression of all the REG family genes in IBD is still unclear. Here, we analyzed expression of all the REG family genes (REGIα, REGIβ, REG III, HIP/PAP, and REG IV) in biopsy specimens of UC and CD by real-time RT-PCR. REG Iα, REG Iβ, and REG IV genes were overexpressed in CD samples. REG IV gene was also overexpressed in UC samples. We further analyzed the expression mechanisms of REG Iα, REG Iβ, and REG IV genes in LS-174T and HT-29 human colonic epithelial cells. The expression of REG Iα was significantly induced by IL-6 or IL-22, and REG Iβ was induced by IL-22. Deletion analyses revealed that three regions (− 220~− 211, − 179~− 156, and − 146~− 130) in REG Iα and the region (− 274~− 260) in REG Iβ promoter were responsible for the activation by IL-22/IL-6. The promoters contain consensus transcription factor binding sequences for MZF1, RTEF1/TEAD4, and STAT3 in REG Iα, and HLTF/FOXN2F in REG Iβ, respectively. The introduction of siRNA for MZF1, RTEF1/TEAD4, STAT3, and HLTF/FOXN2F abolished the transcription of REG Iα and REG Iβ. The gene activation mechanisms of REG Iα/REG Iβ may play a role in colon mucosal regeneration in IBD.

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Authors and Affiliations

  1. Department of Biochemistry, Nara Medical University, Kashihara, 634-8521, Japan

    Shin Takasawa, Chikatsugu Tsuchida, Sumiyo Sakuramoto-Tsuchida, Maiko Takeda, Asako Itaya-Hironaka, Akiyo Yamauchi, Masayasu Misu, Ryogo Shobatake, Tomoko Uchiyama & Mai Makino

  2. Department of Diagnostic Pathology, Nara Medical University, Kashihara, Japan

    Maiko Takeda, Tomoko Uchiyama & Chiho Ohbayashi

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  1. Shin Takasawa
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  2. Chikatsugu Tsuchida
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  3. Sumiyo Sakuramoto-Tsuchida
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  5. Asako Itaya-Hironaka
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Correspondence to Shin Takasawa.

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This work was performed with approval of the Review Board(s) of Saiseikai Nara Hospital and Nara Medical University Hospital.

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The authors declare that they have no conflict of interest.

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Takasawa, S., Tsuchida, C., Sakuramoto-Tsuchida, S. et al. Expression of human REG family genes in inflammatory bowel disease and their molecular mechanism. Immunol Res 66, 800–805 (2018). https://doi.org/10.1007/s12026-019-9067-2

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  • DOI: https://doi.org/10.1007/s12026-019-9067-2

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