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Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity

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Abstract

In the present research, we have studied an influence of enhanced expression TRIM14 on alphavirus Sindbis (SINV, Togaviridae family) infection. In the HEK293 cells transfected with human trim14 gene (HEK–trim14), SINV yield after infection was decreased 1000–10,000 times (3–4 lg of TCD50/ml) at 24 h p.i. and considerably less (1–2 lg of TCD50/ml) at 48 h p.i. Analysis of the expression of 43 genes directly or indirectly involved in innate immune machine in HEK–trim14 non-infected cells comparing with the control (non-transfected) HEK293 cells revealed that stable trim14 transfection in HEK293 cells caused increased transcription of 18 genes (ifna, il6 (ifnβ2), isg15, raf-1, NF-kB (nf-kb1, rela, nf-kb2, relb), grb2, grb3-3, traf3ip2, junB, c-myb, pu.1, akt1, tyk2, erk2, mek2) and lowered transcription of 3 genes (ifnγ, gata1, il-17a). The similar patterns of genes expression observe in SINV-infected non-transfected HEK293 cells. However, SINV infection of HEK–trim14 cells caused inhibition of the most interferon cascade genes as well as subunits of transcription factor NF-κB. Thus, stable enhanced expression of trim14 gene in cells activates the transcription of many immunity genes and suppresses the SINV reproduction, but SINV infection of HEK–trim14 cells promotes inhibition of some genes involved in innate immune system.

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Acknowledgments

This study was supported by Grant Numbers 13-04-00598 and 15-04-07752 from the Russian Foundation for Basic Research and grant for Molecular and Cellular Biology from Russian Academy of Sciences.

Conflict of interest

The authors declare no financial or commercial conflict of interest.

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, 123182, Moscow, Russia

    V. V. Nenasheva, G. V. Kovaleva, N. V. Khaidarova & V. Z. Tarantul

  2. The D.I. Ivanovsky’s Institute of Virology, Ministry of Health, Russia, Moscow, Russia

    L. V. Uryvaev, K. S. Ionova, A. V. Dedova & G. K. Vorkunova

  3. Koblenz-Landau University, Koblenz, Germany

    S. V. Chernyshenko

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  1. V. V. Nenasheva
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Correspondence to V. V. Nenasheva.

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Nenasheva, V.V., Kovaleva, G.V., Uryvaev, L.V. et al. Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity. Immunol Res 62, 255–262 (2015). https://doi.org/10.1007/s12026-015-8653-1

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