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Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia

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Abstract

The aim of this study was to determine chemerin levels in preeclampsia and to assess the effects of this anti-inflammatory factor on endothelial nitric oxide synthase (eNOS), nuclear factor (NF)-κB, and vascular cell adhesion molecule (VCAM) expression in human umbilical vein endothelial cells (HUVECs). Serum chemerin and eNOS levels were measured by enzyme-linked immunosorbent assays, while chemerin mRNA and protein levels were measured by fluorescent quantitative polymerase chain reaction and Western blotting, respectively. Nitric oxide (NO) concentrations were determined with a colorimetric method. Akt and eNOS phosphorylation were assessed by Western blotting. We also tested the effects of the phosphoinositide 3-kinase inhibitor LY294002 and the eNOS inhibitor L-NAME. NF-κB p65 and VCAM-1 phosphorylation were assessed by Western blotting to investigate the role of chemerin in tumor necrosis factor (TNF)-α-induced HUVEC injury. Serum chemerin levels were increased in preeclampsia, while eNOS was decreased. Chemerin mRNA and protein were both increased in placentae from patients with preeclampsia. Furthermore, chemerin serum level positively correlated with blood pressure, body mass index, and serum insulin and was negatively correlated with serum eNOS. Chemerin dose-dependently increased NO concentrations in supernatants. Chemerin can increase eNOS and Akt levels in HUVECs, and these results could be partly blocked by LY294002 and L-NAME. Chemerin significantly decreased TNF-α-induced NF-κB and VCAM-1 in HUVECs, and these changes were partly inhibited by LY294002 and L-NAME. Chemerin may play a protective role by regulating NO signaling. Future studies should assess the role of chemerin in preeclampsia and other vascular diseases.

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Acknowledgments

The authors are grateful to the staff of the Division of Obstetrics and Gynaecology at the Second Xiangya Hospital of Central South University for their assistance and collaboration in data collection.

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We declare that we have no conflicts of interests.

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All registries have ethical approval appropriate to their national and local ethics guidelines.

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Authors and Affiliations

  1. Department of Gynaecology and Obstetrics, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China

    Liqiong Wang

  2. Department of Obstetrics and Gynaecology, The Second XiangYa Hospital of Central South University, 139 Ren Min Zhong Lu, Changsha, Hunan, 410011, China

    Tianli Yang, Yiling Ding, Yan Zhong, Ling Yu & Mei Peng

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  1. Liqiong Wang
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  2. Tianli Yang
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  3. Yiling Ding
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Correspondence to Yiling Ding.

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Wang, L., Yang, T., Ding, Y. et al. Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia. Endocrine 48, 299–308 (2015). https://doi.org/10.1007/s12020-014-0286-y

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  • DOI: https://doi.org/10.1007/s12020-014-0286-y

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