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Thyroid hormones regulate skeletal muscle regeneration after acute injury

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Abstract

We evaluated the effects of hypo- and hyperthyroid statuses during the initial phase of skeletal muscle regeneration in rats. To induce hypo- or hyperthyroidism, adult male Wistar rats were treated with methimazole (0.03 %) or T4 (10 μg/100 g), respectively, for 10 days. Three days before sacrifice, a crush injury was produced in the solear muscles of one half of the animals, while the other half remained intact. T3, T4, TSH, and leptin serum levels were not affected by the injury. Serum T3 and T4 levels were significantly increased in hyperthyroid and hyper-injury animals. Hypothyroidism was confirmed by the significant increase in serum TSH levels in hypothyroid and hypo-injury animals. Injury increased cell infiltration and macrophage accumulation especially in hyperthyroid animals. Both type 2 and type 3 deiodinases were induced by lesion, and the opposite occurred with the type 1 isoform, at least in the control and hyperthyroid groups. Injury increased both MyoD and myogenin expression in all the studied groups, but only MyoD expression was increased by thyroidal status only at the protein level. We conclude that thyroid hormones modulate skeletal muscle regeneration possibly by regulating the inflammatory process, as well as MyoD and myogenin expression in the injured tissue.

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Acknowledgments

The authors gratefully acknowledge the technical assistance of Advaldo Nunes Bezerra, José Humberto Tavares de Abreu, Norma Lima de Araújo Faria, and Wagner Nunes Bezerra. This work was supported by the grants from Fundação Carlos Chagas Filho de Amparo `a Pesquisa do Estado do Rio de Janeiro (FAPERJ), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors declare no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, bloco G, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, 21949-900, Brazil

    Anna Lúcia R. C. Leal, João Paulo C. Albuquerque, Marina S. Matos, Rodrigo S. Fortunato, Denise P. Carvalho, Doris Rosenthal & Vânia Maria Corrêa da Costa

  2. VM Corrêa da Costa, Instituto de Biofísica Carlos Chagas Filho, CCS Bloco G, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, 21949-900, Brazil

    Vânia Maria Corrêa da Costa

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  1. Anna Lúcia R. C. Leal
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Correspondence to Vânia Maria Corrêa da Costa.

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Leal, A.L.R.C., Albuquerque, J.P.C., Matos, M.S. et al. Thyroid hormones regulate skeletal muscle regeneration after acute injury. Endocrine 48, 233–240 (2015). https://doi.org/10.1007/s12020-014-0271-5

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