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Uncovering the Cellular Microenvironment in Chronic Rhinosinusitis via Single-Cell RNA Sequencing: Application and Future Directions

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Abstract

Chronic rhinosinusitis (CRS) is a heterogenic disease characterized by persistent mucosal inflammation of the upper airway. Researches of CRS have progressed from phenotype-based to endotype-based, looking more deeply into molecular biomarkers, signaling pathways, and immune microenvironment. Single-cell RNA sequencing is an effective tool in analyzing composition, function, and interaction of cells in disease microenvironment at transcriptome level, showing great advantage in analyzing potential biomarkers, pathogenesis, and heterogeneity of chronic airway inflammation in an unbiased manner. In this article, we will review the latest advances in scRNA-seq studies of CRS to provide new perspectives for the diagnosis and treatment of this heterogeneous disease.

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Funding

This work was funded by the National Natural Science Foundation of China (grant numbers 82071027 and 82101200); National High Level Hospital Clinical Research Funding (grant numbers 2022-PUMCH-B-096, 2022-PUMCH-A-030, and 2022-PUMCH-C-050).

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  1. Department of Otorhinolaryngology-Head and Neck Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100730, China

    Yuzhuo Liu, Wei Lv & Weiqing Wang

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  1. Yuzhuo Liu
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  2. Wei Lv
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All authors contributed to the study conception and design. Literature search and data analysis were performed by Yuzhuo Liu and Weiqing Wang. The first draft of the manuscript was written by Yuzhuo Liu, and all authors revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wei Lv or Weiqing Wang.

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Liu, Y., Lv, W. & Wang, W. Uncovering the Cellular Microenvironment in Chronic Rhinosinusitis via Single-Cell RNA Sequencing: Application and Future Directions. Clinic Rev Allerg Immunol (2024). https://doi.org/10.1007/s12016-024-08992-6

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