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Combining Bone Collagen Material with hUC-MSCs for Applicationto Spina Bifida in a Rabbit Model

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Abstract

Spina bifida is one of the neural tube defects, with a high incidence in human birth defects, which seriously affects the health and quality of life of patients. In the treatment of bone defects, the source of autologous bone is limited and will cause secondary damage to the patient. At the same time, since the bone tissue in animals needs to play a variety of biological functions, its complex structure cannot be replaced by a single material. The combination of mechanical materials and biological materials has become a common choice. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have the advantages of easy access, rapid proliferation, low immunogenicity, and no ethical issues. It is often used in the clinical research of tissue regeneration and repair. Therefore, in this study, we established a spina bifida model using Japanese white rabbits. This model was used to screen the best regenerative repair products for congenital spina bifida, and to evaluate the safety of regenerative repair products. The results showed that the combination of hUC-MSCs with collagen material had better regeneration effect than collagen material alone, and had no negative impact on the health of animals. This study provides a new idea for the clinical treatment of spina bifida, and also helps to speed up the research progress of regenerative repair products.

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Data Availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

The authors are very grateful to the animal experiment center of the National Research Institute for Family Planning for its meticulous care of animals.

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by grants from the National Key Research and Development Program of China [2016YFC1000803].

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Author notes

  1. Hu Wang and Xue-Cheng Sun that authors contribute equally to this work.

Authors and Affiliations

  1. Reproductive and Genetic Center, NHC Key Laboratory of Reproductive Health Engineering Technology Research (NRIFP), National Research Institute for Family Planning, Beijing, 100081, China

    Hu Wang, Xue-Cheng Sun, Jian-Hui Li, Xu Ma & Hong-Fei Xia

  2. Medical Genetics, Zibo Maternal and Child Health Hospital, Zibo, 255000, China

    Xue-Cheng Sun

  3. Graduate Schools, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, 100730, China

    Hu Wang, Xu Ma & Hong-Fei Xia

  4. Yantai Zhenghai Bio-Tech Co., Ltd., Yantai, 264006, China

    Li-Qiang Yin & Yu-Fang Yan

Authors
  1. Hu Wang
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  3. Jian-Hui Li
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  7. Hong-Fei Xia
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Contributions

XCS, XM and HFX designed the study. HW, XCSand JHL were responsible for the vivo surgery. XCS and HW were responsible for in vitro experiments. HW,XCS and HFX prepared the manuscript. YFY and LQY provided the bone repair materials and collagen membranes. HW, XCS, JHL and HFX were responsible for revising the manuscript critically for important intellectual content. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Xu Ma or Hong-Fei Xia.

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Conflict of Interest

The authors declare that they have no competinginterests.

Ethical Approval

Ethical approval to report this case was obtained fromthe National Research Institute for Family Planning (Ethics Number2015-16).

Consent for Publication

All authors gave consent for publication.

Informed Consent

There are no human subjects in this article andinformed consent is not applicable.

Research involving Human and Animal Rights

All procedures in thisstudy were conducted in accordance with the National Research Institutefor Family Planning (Ethics Number 2015–16) approved protocols.

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Wang, H., Sun, XC., Li, JH. et al. Combining Bone Collagen Material with hUC-MSCs for Applicationto Spina Bifida in a Rabbit Model. Stem Cell Rev and Rep 19, 1034–1050 (2023). https://doi.org/10.1007/s12015-022-10478-x

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  • DOI: https://doi.org/10.1007/s12015-022-10478-x

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