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INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats

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Abstract

The parotid gland is the largest salivary gland. It produces watery saliva, rich in proteins (amylase, lysozymes, and antibodies). Due to the gland’s morphological cytoarchitecture composed of only serous acini, it contributes almost 50% of total salivary volume upon stimulation. It has been reported that the prevalence of saliva secretion impairments, periodontitis, delayed wound healing, and xerostomia increase in diabetic patients. Herein we evaluated the acute effects of insulin on insulin receptor phosphorylation status and its substrates IRS-1 and IRS-2 in the parotid glands of adult male Wistar rats, using Western blot analyses. We confirmed an acute effect of insulin on IR/IRS/PI3K/Akt and MAPK intracellular pathway activation in the parotid glands of male Wistar rats similar to the classical metabolic targets of the hormone, like the liver.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 1524, São Paulo, SP, 05508-000, Brazil

    André Guaraci DeVito-Moraes, Victor Di Donato Marques, Luciana Chagas Caperuto & Carla Roberta de Oliveira Carvalho

  2. Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2227, São Paulo, SP, 05508-000, Brazil

    André Guaraci DeVito-Moraes, Flavia Kazue Ibuki, Fernando Neves Nogueira & Carlos Eduardo Francci

  3. Discipline of Dental Biomaterials, School of Dentistry, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249, São Paulo, SP, 01504-001, Brazil

    André Guaraci DeVito-Moraes

  4. Department of Biological Sciences, Federal University of São Paulo (UNIFESP), R. Prof. Artur Riedel, 275, Diadema, SP, 09972-270, Brazil

    Luciana Chagas Caperuto

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  1. André Guaraci DeVito-Moraes
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Correspondence to André Guaraci DeVito-Moraes.

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DeVito-Moraes, A.G., Marques, V.D.D., Caperuto, L.C. et al. INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats. Cell Biochem Biophys 80, 89–95 (2022). https://doi.org/10.1007/s12013-021-01025-5

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