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Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells

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Abstract

The vitamin E derivative with side chain modification (TC6OAc) has been shown to possess anticancer activity in our earlier in vivo studies. It was hypothesized that, as Vitamin E (VE) and VE derivative are fat soluble lipophilic molecules, they exert their function by modulating the lipid metabolism and related pathways. This study aimed to evaluate the cellular impact of this VE derivative (2,5,7,8-Tetramethyl-2-(4′-Methyl-3′-Pentenyl)-6-Acetoxy Chromane-TC6OH), using α-tocopherol as a reference compound throughout the experiments. Their effects on the cellular metabolism, the biophysical properties of cellular lipids and the functional characteristics of cells were monitored in human estrogen receptor (ER) positive breast cancer cells. It has been documented that TC6OH treatment induces tumor cell apoptosis by dissipating the mitochondrial membrane potential, modulating the lipid, transportation and degradation as well as downregulating certain anti-apoptotic and growth factor related proteins. Due to resistance of ER positive cells to the established therapies, the findings of this study are of translational value.

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Acknowledgements

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 114Z424) and the National Academy of Sciences of Ukraine for this joint research project.

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Authors and Affiliations

  1. The Scientific and Technological Research Council of Turkey, Ankara, Turkey

    Seher Gok

  2. Department of Vitamins and Coenzymes Biochemistry, Palladin Institute of Biochemistry, Kiev, Ukraine

    Oleksandr Kuzmenko & Andrii Babinskyi

  3. Faculty of Medicine, Department of Biophysics, Altinbas University, Istanbul, Turkey

    Feride Severcan

  4. Department of Biological Sciences, Middle East Technical University, Ankara, Turkey

    Feride Severcan

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  1. Seher Gok
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  2. Oleksandr Kuzmenko
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Gok, S., Kuzmenko, O., Babinskyi, A. et al. Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells. Cell Biochem Biophys 79, 271–287 (2021). https://doi.org/10.1007/s12013-020-00961-y

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