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Biophysical Aspect of Huntingtin Protein During polyQ: An In Silico Insight

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Abstract

Huntington’s disease (HD) is a neurodegenerative disorder that is caused by an abnormal elongation of the polyglutamine (polyQ) chain in the Huntington (Htt) protein. At present, the normal function of Htt of neurons as well as the mechanism by which selective neurodegeneration is caused by the expanded polyQ chain in Htt remains ambiguous. A gain of function as a result of the elongated polyQ chain can lead to abnormal interaction of the Htt protein with its interacting partners, thereby resulting in the neuropathological changes seen in the Huntington’s disease. Recent research indicates protein kinase C and casein kinase substrate in neurons protein 1 (PACSIN1) as one of the interacting partners of Htt protein. It has proven experimentally that the mutant Htt and PACSIN1 formed aggregates in the cytoplasm. This aggregation is believed to be a cause for Huntington’s disease. In our study, we performed in silico investigations to predict the biomolecular mechanism of Htt/PACSIN1 interaction that could be one of the major triggers of the disease. Biomolecular interaction and molecular dynamics simulation analysis were performed to understand the dynamic behavior of native and mutant structures at the atomic level. Mutant Htt showed more interaction with its biological partner than the native Htt due to its expansion of interaction surface and flexible nature of binding residues. Our investigation of native and mutant Htt clearly shows that the structural and functional consequences of the polyQ elongation cause HD. Because of the central role of the Htt-PACSIN1 complex in maintaining connections between neurons, these differences likely contribute to the mechanism responsible for HD progression.

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Acknowledgments

We gratefully acknowledge the CSIR-Institute of Himalayan Bioresource Technology, Palampur, Vellore Institute of Technology University and Bioinformatics Resources and Applications Facility (BRAF), C-DAC, Pune for providing the facilities to carry out this work. This manuscript represents CSIR-IHBT communication number 4022.

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    Authors and Affiliations

    1. Computational Biology Lab, Department of Biotechnology, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India

      Chandrasekhar Gopalakrishnan, Shraddha Jethi & Namrata Kalsi

    2. Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India

      Rituraj Purohit

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    1. Chandrasekhar Gopalakrishnan
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    Chandrasekhar Gopalakrishnan and Shraddha Jethi have contributed equally to this manuscript.

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    Gopalakrishnan, C., Jethi, S., Kalsi, N. et al. Biophysical Aspect of Huntingtin Protein During polyQ: An In Silico Insight. Cell Biochem Biophys 74, 129–139 (2016). https://doi.org/10.1007/s12013-016-0728-7

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