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Down-Regulation of MRP1 Expression in C6/VP16 Cells by Chitosan-MRP1-siRNA Nanoparticles

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Abstract

High expression of multidrug resistance-associated protein 1 (MRP1) in tumor cells reduces effectiveness of chemotherapy drugs. In this study, we screened MRP1 interfering RNA (MRP1-siRNA) molecules that are able to reverse etoposide (VP16) resistance in multidrug resistance rat glioma cell line C6/VP16, and identified one siRNA molecule that is able to effectively deplete the expression of MRP1 gene and reverse tumor cells resistance to etoposide. Since siRNA instability limits its application in treatment of diseases, we next tested silencing effect of chitosan-MRP1-siRNA nanoparticles and found that the nanoparticles with N:P ratio 175 are able to effectively inhibit MRP1 mRNA and protein expression. Our data demonstrate that chitosan can be used as siRNA carrier for high efficient gene silencing in tumor cells.

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Authors and Affiliations

  1. Department of Neurosurgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan, 430060, China

    Haitao Xu, Xiaoqi Nie, Liquan Wu, Xiaonan Zhu, Wei Yi & Shulan Huang

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  1. Haitao Xu
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  2. Xiaoqi Nie
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  3. Liquan Wu
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  4. Xiaonan Zhu
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  5. Wei Yi
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  6. Shulan Huang
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Correspondence to Shulan Huang.

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Xu, H., Nie, X., Wu, L. et al. Down-Regulation of MRP1 Expression in C6/VP16 Cells by Chitosan-MRP1-siRNA Nanoparticles. Cell Biochem Biophys 72, 227–233 (2015). https://doi.org/10.1007/s12013-014-0442-2

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  • DOI: https://doi.org/10.1007/s12013-014-0442-2

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