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Protective Effects of Sesaminol on BEAS-2B Cells Impaired by Cigarette Smoke Extract

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Abstract

The aim of this study is to investigate protective effects of sesaminol on the human bronchial epithelial (BEAS-2B) cell line against oxidative damage of cigarette smoke extract (CSE). BEAS-2B cells were pre-incubated with sesaminol for 12 h and then treated with various concentrations of CSE for 24 h. After that proliferation ability, levels of reactive oxygen species (ROS) and lactate dehydrogenase (LDH), cell apoptosis, activities of catalase (CAT) and superoxide dismutase (SOD), and mRNA levels of IL-8 and IL-6 were measured. The results showed that sesaminol significantly improved BEAS-2B cell viability, reduced the production of ROS and LDH of cells, inhibited cell apoptosis and increased CAT and SOD activities in CSE-treated cells. Sesaminol also inhibited the expression of IL-8 and IL-6 mRNA following CSE exposure. In conclusion, sesaminol may protect BEAS-2B cells against CSE-induced oxidative damage.

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Acknowledgments

This study was financed by the National Natural Science Foundation of China (Nos. 81273121 and 81070839).

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Authors and Affiliations

  1. State Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University Medical School, 22 Hankou Road, Nanjing, 210093, China

    Ping Dong, Xiao Fu, Xiang Wang & Wei-yun Zhang

  2. Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, 21008, China

    Xiang Wang & Wen-mei Wang

  3. Shanghai Grain Science Research Institute, Shanghai, 200136, China

    Wen-ming Cao

Authors
  1. Ping Dong
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  2. Xiao Fu
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  3. Xiang Wang
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  4. Wen-mei Wang
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  5. Wen-ming Cao
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  6. Wei-yun Zhang
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Correspondence to Wen-ming Cao or Wei-yun Zhang.

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Dong, P., Fu, X., Wang, X. et al. Protective Effects of Sesaminol on BEAS-2B Cells Impaired by Cigarette Smoke Extract. Cell Biochem Biophys 71, 1207–1213 (2015). https://doi.org/10.1007/s12013-014-0330-9

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  • DOI: https://doi.org/10.1007/s12013-014-0330-9

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