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Modulatory Effect of Aerobic Exercise Training on Doxorubicin-Induced Cardiotoxicity in Rats with Different Ages

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Abstract

We tested the hypothesis that aerobic exercise training (AET) would modulate doxorubicin-induced cardiotoxicity in rats of various ages. Wistar male rats (n = 72) were assigned to three groups (young, adult, and elderly) with three subgroups for each age: doxorubicin (DG, n = 8), AET + doxorubicin (AETDG, n = 8), AET + Saline (AETSG, n = 8). Following the AET intervention, rats were anesthetized and killed to collect heart tissues in order to determine heat shock protein 70 (HSP70), superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-10 (IL10), and c-reactive protein (CRP). Greater levels of SOD and lower levels of MDA were found in young as compared to elderly rats (P < 0.05). CRP was significantly lower in young as compared to adult and elderly rats, respectively (P < 0.05). IL10 also was significantly lower in young as compared to elderly rats (P < 0.05). Furthermore, lower HSP70 and SOD levels were found in DG as compared to AETDG and AETSG (P < 0.05). Conversely, MDA and CRP were significantly higher in DG as compared to other groups (P < 0.05). A significant higher IL10 was noted in DG as compared to AETDG (P < 0.05). Our findings suggest that AET prior to doxorubicin treatment could be useful in minimizing toxicity after chemotherapy with doxorubicin mainly up-regulating of antioxidant defense capacity, with greatest benefit in the young group. Future studies examining the exercise-induced changes and age-related differences following chemotherapy are necessary in human.

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Acknowledgements

The authors would like to thank Dr. Christopher West (University of British Columbia) for his helpful comments on the manuscript.

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Authors and Affiliations

  1. Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

    Mehdi Ahmadian

  2. College of Physical Education and Sport Sciences, Department of Sport Physiology, University of Mazandaran (UMZ), Pasdaran Street, 47415, P.O. Box: 416, Babolsar, Mazandaran, Iran

    Valiollah Dabidi Roshan

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Correspondence to Valiollah Dabidi Roshan.

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Ahmadian, M., Dabidi Roshan, V. Modulatory Effect of Aerobic Exercise Training on Doxorubicin-Induced Cardiotoxicity in Rats with Different Ages. Cardiovasc Toxicol 18, 33–42 (2018). https://doi.org/10.1007/s12012-017-9411-5

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