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Ameliorative Effects of Zn and Se Supplementation on Heavy Metal Mixture Burden via Increased Renal Metal Excretion and Restoration of Redoxo-Inflammatory Alterations

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Abstract

Heavy metals (HM)in the environment have provoked global attention because of its deleterious effects. This study evaluated the protection offered by Zn or Se or both against HMM-induced alterations in the kidney. Male Sprague Dawley rats were distributed into 5 groups of 7 rats each. Group I served as normal control with unrestricted access to food and water. Group II received Cd, Pb, and As (HMM) per oral daily for 60 days while groups III and IV received HMM in addition to Zn and Se respectively for 60 days. Group V received both Zn and Se in addition to HMM for 60 days. Metal accumulation in feces was assayed at days 0, 30, and 60 while accumulation in the kidney and kidney weight were measured at day 60. Kidney function tests, NO, MDA, SOD, catalase, GSH, GPx, NO, IL-6, NF-Κb, TNFα, caspase 3, and histology were assessed. There is a significant increase in urea, creatinine, and bicarbonate ions while potassium ions decreased. There was significant increase in renal function biomarkers, MDA, NO, NF-Κb, TNFα, caspase 3, and IL-6 while SOD, catalase, GSH, and GPx decrease. Administration of HMM distorted the integrity of the rat kidney, and co-treatment with Zn or Se or both offered reasonable protection suggesting that Zn or Se could be used as an antidot against the deleterious effects of these metals.

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Authors and Affiliations

  1. African Centre of Excellence for Public Health and Toxicological Research (ACE-PUTOR), University of Port Harcourt, Port Harcourt, Choba, PMB, 5323, Nigeria

    Harrison Ozoani, Anthonet N. Ezejiofor & Orish E. Orisakwe

  2. Department of Pharmacology & Toxicology, Faculty of Pharmacy, Enugu State, University of Science & Technology, Enugu, Nigeria

    Harrison Ozoani & Kenneth O. Okolo

  3. Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Port Harcourt, Choba, PMB, 5323, Nigeria

    Chinna N. Orish

  4. University of Belgrade, Faculty of Medicine, Institute of Anatomy, Belgrade, Serbia

    Ana Cirovic & Aleksandar Cirovic

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  1. Harrison Ozoani
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HO, ANE, CNO Bench work, Data Sampling and data acquisition; KOO, AC, AC Data analyses and drafting of manuscript, OEO Conceptualization and drafting of manuscript.

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Correspondence to Orish E. Orisakwe.

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Ozoani, H., Ezejiofor, A.N., Okolo, K.O. et al. Ameliorative Effects of Zn and Se Supplementation on Heavy Metal Mixture Burden via Increased Renal Metal Excretion and Restoration of Redoxo-Inflammatory Alterations. Biol Trace Elem Res 202, 643–658 (2024). https://doi.org/10.1007/s12011-023-03709-w

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