Abstract
It is known that boric acid (BA) exerts it antioxidant and anti-inflammatory effects by activating the activating transcription factor 4 (ATF4) and nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway. This pathway has been reported to control antioxidant status in the eye. The aim of this study was to investigate the possible preventive effects of boric acid administration on oxidative damage and corneal neovascularization (CNV). Sixteen adult female Wistar albino rats were divided into two groups: (I) control (n = 8); the CNV model was applied to the right eye of the rats, and the left eyes were used as healthy controls. (II) CNV + BA (n = 8): After the CNV model was applied to the right eyes, a single subconjunctival dose (0.05 mL) of 0,018 g/mL BA was injected into the right and left eyes of the rats. Biochemical, histopathological, and immunohistochemical analyses were performed. Moderate VEGF positivity was observed in the vessels of the CNV group, a decrease in vessel proliferation, and weak VEGF positivity in the CNV + BA group. The TAS level in the CNV + BA group was significantly higher than that in the other groups. The TOS level was significantly higher in all groups than it is in the control group. The OSI value was increased in all groups when compared to the control group, but only the CNV and BA groups were statistically significant. BA not only reduced alkaline-induced corneal damage histologically but also showed a protective effect on oxidative stress biochemically.
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The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request. Unfortunately, the data are not publicly available because of local data protection laws.
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This study was supported by the Scientific Research Fund of the Suleyman Demirel University with the project number TSG-2020–8134.
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Study conceptualization: (UK, MS); Conduct of study (UK, SGP, MS, MA, GU, DO), Data curation (UK, İİ), Formal analysis (UK, MS, İİ), Writing-original draft (UK, SGP, MS), Writing-review and editing (All Authors).
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All experiments were performed in accordance with the guidelines for animal research from the National Institutes of Health and approved by the Committee on Animal Research of Suleyman Demirel University, Isparta (No: 03.09.2020–05/12).
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Karaca, U., Pinar, S.G., Savran, M. et al. The Possible Protective Effect of Boric Acid in an Alkaline-Induced Corneal Neovascularization Rat Model. Biol Trace Elem Res 200, 5127–5133 (2022). https://doi.org/10.1007/s12011-022-03094-w
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DOI: https://doi.org/10.1007/s12011-022-03094-w