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Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

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Abstract

In this study, the protective effects of chrysin (CR) on lead acetate (PbAc)-induced renal toxicity in Sprague-Dawley rats were investigated with biochemical, histopathological, and immunohistochemical methods. In the study, rats were given orally at 30 mg/kg/body weight (BW) PbAc after CR of 25 and 50 mg/kg/BW was administered to them orally (a total of 7 administrations for 7 days). The results showed that CR reduced urea and creatinine levels by alleviating PbAc-induced kidney damage. It was determined that CR decreases PbAc-induced lipid peroxidation due to its antioxidant properties and increases catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) levels. It was also detected that CR protects DNA from the toxic effects of PbAc and reduces 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. Biochemical and immunohistochemical findings demonstrated that CR had anti-inflammatory and antiapoptotic effects and reduced nuclear factor kappa-B (NF-κB), interleukin-33 (IL-33), prostaglandin-E2 (PGE-2), tumor necrosis factor-α (TNF-α), p53 levels, and the activities of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), which were increased with PbAc administration. Moreover, CR was found to increase the levels of aquaporin-1 (AQP-1) and nephrine in PbAc-induced kidney tissue. CR decreased the contents of lead (Pb), zinc (Zn), iron (Fe), sodium (Na), and copper (Cu) and increased those of potassium (K) calcium (Ca) in renal tissue. These results indicated that CR considerably alleviates kidney toxicity caused by PbAc.

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Funding

This study was funded by the Unit of Scientific Research Projects in Munzur University. (Project No: YLMUB017-24)

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey

    Sefa Kucukler, Cihan Gur & Fatih Mehmet Kandemir

  2. Department of Midwifery, Faculty of Health Science, Munzur University, 62000, Tunceli, Turkey

    Fulya Benzer

  3. Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey

    Serkan Yildirim & Muhammet Bahaeddin Dortbudak

  4. Department of Medical Services and Tecniques, Program of Medical Laboratory Tecniques, Bingol University, 12000, Bingöl, Turkey

    Aydin Sukru Bengu

  5. Department of Chemistry, Faculty of Sciences and Arts, Bingol University, 12000, Bingöl, Turkey

    Adnan Ayna

  6. Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, 12000, Bingöl, Turkey

    Cuneyt Caglayan

Authors
  1. Sefa Kucukler
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  2. Fulya Benzer
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  3. Serkan Yildirim
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  4. Cihan Gur
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  5. Fatih Mehmet Kandemir
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  6. Aydin Sukru Bengu
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  7. Adnan Ayna
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  8. Cuneyt Caglayan
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  9. Muhammet Bahaeddin Dortbudak
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Corresponding author

Correspondence to Fatih Mehmet Kandemir.

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Kucukler, S., Benzer, F., Yildirim, S. et al. Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach. Biol Trace Elem Res 199, 1501–1514 (2021). https://doi.org/10.1007/s12011-020-02268-8

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  • DOI: https://doi.org/10.1007/s12011-020-02268-8

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