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Serum CRP, MDA, Vitamin C, and Trace Elements in Bangladeshi Patients with Rheumatoid Arthritis

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Abstract

Rheumatoid arthritis (RA) is an autoimmune disorder that is a painful health crisis. This study aimed to assess the serum C-reactive protein (CRP), malondialdehyde (MDA), non-enzymatic antioxidant (vitamin C), and trace elements (Zn, Cu, Mn, and Fe) in RA patients, and thereby correlate these parameters with the association of RA. This study included 20 Bangladeshi RA patients and 20 normal healthy volunteers as control subjects. CRP level was determined using a laboratory-based latex agglutination–enhanced immunoassay. The lipid peroxidation level was determined by measurement of the serum level of MDA. Non-enzymatic antioxidant vitamin C was assessed by UV spectrophotometric method. Trace elements were determined by atomic absorption spectroscopy (AAS). Our study observed significantly higher concentrations of CRP (p < 0.001) and MDA (p < 0.001), and significantly lower concentrations of vitamin C (p < 0.001) in the RA patient. The mean values of Zn, Cu, Mn, and Fe were 6.62 ± 0.34, 1.42 ± 0.17, 7.51 ± 0.23, and 29.25 ± 0.41 ppm for the RA patients respectively and 13.57 ± 9.13, 1.15 ± 0.17, 1.59 ± 0.18, and 62.47 ± 5.25 ppm for the control subjects, consequently. There was a significant difference (p < 0.05) in the trace element levels between the RA patients and control subjects. Our study suggests that a higher concentration of CRP and MDA, lower levels of vitamin C, and altered trace elements may be linked to RA.

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Abbreviations

RA:

rheumatoid arthritis

CRP:

C-reactive protein

MDA:

malondialdehyde

ROS:

reactive oxygen species

BMI:

body mass index

SBP:

systolic blood pressure

DBP:

diastolic blood pressure

FAAS:

flame atomic absorption spectrometry

HPLC:

high-performance liquid chromatography

TBA:

thiobarbituric acid

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Acknowledgments

The authors are thankful to all the staff and physicians at the Dhaka Medical College and Hospital (DMCH), Dhaka, Bangladesh, for their generous help during sample collection. The authors are also thankful for the technical and laboratory support provided by Agrochemical and Environmental Research Division, Institute of Food and Radiation Biology, Bangladesh Atomic Energy Research Establishment, Dhaka. The authors express their gratitude to all the staff and faculty members of the Department of Pharmacy, Noakhali Science and Technology University, Bangladesh, for providing laboratory facilities.

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Authors and Affiliations

  1. Department of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh

    Dalim Chandra Das, Israt Jahan & Abhijit Das

  2. Department of Pharmacy, University of Chittagong, Chittagong, 4331, Bangladesh

    Md. Giash Uddin

  3. Department of Pharmacy, Mawlana Bhashani Science and Technology University, Santosh, Tangail, 1902, Bangladesh

    Md. Monir Hossain

  4. Agrochemical and Environmental Research Division, Institute of Food and Radiation Biology, Bangladesh Atomic Energy Research Establishment, Savar, Dhaka, 1349, Bangladesh

    Muhammed Alamgir Zaman Chowdhury & Zeenath Fardous

  5. Department of Medicine, Dhaka Medical College and Hospital, Dhaka, 1000, Bangladesh

    Md. Mujibur Rahman, A. K. M. Humayun Kabir, Sudip Ranjan Deb & Md. Abu Bakar Siddique

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  1. Dalim Chandra Das
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Contributions

DCD, IJ, and MGU participated in protocol designing and writing. MMH, MAZC, ZF, MMR, AKMHK, SRD, and MABS contributed in drafting and editing. AD checked and approved the overall manuscript.

Corresponding author

Correspondence to Abhijit Das.

Ethics declarations

This case-control study is carried out in the Department of Medicine, Dhaka Medical College and Hospital (DMCH), Dhaka. The ethical committee of the DMCH approved the research protocol and gave ethical permission (reference no: MEU-DMC/ECC/2016/160).

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The authors declare that they have no conflict of interest.

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Das, D.C., Jahan, I., Uddin, M.G. et al. Serum CRP, MDA, Vitamin C, and Trace Elements in Bangladeshi Patients with Rheumatoid Arthritis. Biol Trace Elem Res 199, 76–84 (2021). https://doi.org/10.1007/s12011-020-02142-7

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