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Hemolysis Is Responsible for Elevation of Serum Iron Concentration After Regular Exercises in Judo Athletes

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Abstract

Serum iron concentration increases in marathon athletes after running due to mechanical destruction of red blood cells (hemolysis). This study was performed to examine whether serum iron concentration increases after regular Judo exercise, and if so, whether such post-exercise iron increase is caused by hemolysis. We examined biochemical parameters related to red blood cell and iron metabolism in 16 male competitive Judo athletes before and after traditional exercise training composed of basic movements and freestyle matchup. The parameters were adjusted for changes in plasma volume based on simultaneously measured albumin concentration. The red blood cell count, hemoglobin concentration, and hematocrit levels decreased significantly, by 6.0–8.4%, after Judo exercise. The serum iron concentration and transferrin saturation increased significantly, from 87 ± 34 μg/dL to 98 ± 29 μg/dL and from 27.1 ± 9.7% to 31.2 ± 9.0%, respectively. Furthermore, the serum free hemoglobin level increased by 33.9% (p < 0.05), and haptoglobin concentration decreased by 19.2% (p < 0.001). A significant negative correlation was observed between Δ haptoglobin concentration and Δ serum iron concentration (r = − 0.551, p = 0.027). The results of this study indicate that serum iron concentration increases significantly after Judo exercise due to hemolysis.

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Abbreviations

BW:

Body weight

CK:

Creatine kinase

CK-MB:

CK muscle-brain

ECLIA:

Electrochemiluminescence immunoassay

free-Hb:

Free hemoglobin

Hb:

Hemoglobin

Ht:

Hematocrit

MCH:

Mean corpuscular hemoglobin

MCHC:

Mean corpuscular hemoglobin concentration

MCV:

Mean corpuscular volume

MPV:

Mean platelet volume

MW:

Molecular weight

PDW:

Platelet distribution width

Plt:

Platelet

RBC:

Red blood cell

RDW-CV:

Red cell distribution width

SD:

Standard deviation

TAST:

Transferrin saturation

TIBC:

Total iron-binding capacity

UIBC:

Unsaturated iron-binding capacity

WBC:

White blood cell

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Funding

This study was supported by a grant from High Technology Research Center Grant, Strategic Research Foundation at private universities (SH and TM).

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Authors and Affiliations

  1. Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo, 113-8421, Japan

    Rina Nishiie-Yano, Satoshi Hirayama, Takumi Kanemochi, Tsuyoshi Ueno, Akiko Hirayama, Atsushi Hori, Tomohiko Ai & Takashi Miida

  2. Department of Sports Science, Juntendo University Graduate School of Health and Sports Science, Hiragagakuendai 1-1, Inzai, Chiba, 270-1695, Japan

    Masahiro Tamura, Takumi Kanemochi & Nobuyoshi Hirose

  3. Toho Junior and Senior High School, Naka 3-1-10, Kunitachi, Tokyo, 186-0004, Japan

    Takumi Kanemochi

  4. Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center, Shinsuna 3-3-20, Koto-ku, Tokyo, 136-0075, Japan

    Tsuyoshi Ueno

  5. Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Hongo 2–1–1, Bunkyo-ku, Tokyo, 113–8421, Japan

    Atsushi Hori

Authors
  1. Rina Nishiie-Yano
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  2. Satoshi Hirayama
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  3. Masahiro Tamura
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  9. Nobuyoshi Hirose
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  10. Takashi Miida
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Correspondence to Satoshi Hirayama.

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Nishiie-Yano, R., Hirayama, S., Tamura, M. et al. Hemolysis Is Responsible for Elevation of Serum Iron Concentration After Regular Exercises in Judo Athletes. Biol Trace Elem Res 197, 63–69 (2020). https://doi.org/10.1007/s12011-019-01981-3

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