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Boron, a Trace Mineral, Alleviates Gentamicin-Induced Nephrotoxicity in Rats

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Abstract

The present study was considered to assess the protective effects of boron (B) on gentamicin-induced oxidative stress, proinflammatory cytokines, and histopathological changes in rat kidneys. Rats were split into eight equal groups which were as follows: control (fed with low-boron diet); gentamicin group (100 mg/kg, i.p.); B5, B10, and B20 (5, 10, and 20 mg/kg B, i.p.) groups; gentamicin (100 mg/kg, i.p.) plus B5, B10, and B20 (5, 10, and 20 mg/kg B, i.p.) groups. B was given to rats 4 days before the gentamicin treatment and B administration was completed on the 14th day. Gentamicin administration was started on the 4th day and finished on the 12th day. Gentamicin increased malondialdehyde levels, while reduced glutathione levels in the blood and kidney. Furthermore, superoxide dismutase and catalase activities of erythrocyte were decreased. Besides, serum and kidney nitric oxide and 8-dihydroxyguanidine levels were increased by gentamicin. Additionally, serum levels and kidney mRNA expressions of TNF-α, NFκB, IL-1β, and IFN-γ were found to be the highest in the gentamicin group. Histopathologically, interstitial hemorrhage and tubular necrosis were detected in the kidneys of the gentamicin group. Nonetheless, B administration reversed gentamicin-induced lipid peroxidation, antioxidant status, and inflammation. In conclusion, B has a preventive effect against gentamicin-induced nephrotoxicity and ameliorates kidney tissues of the rat.

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Acknowledgment

Also, this study orally presented at 3rd International Turkic World Conference on Chemical Sciences and Technologies (ITWCCST 2017).

Funding

This study was financially supported by a grant (Project Number: 214O661) from the Scientific and Technological Research Council of Turkey.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyon Kocatepe University, TR-03200, Afyonkarahisar, Turkey

    Sinan Ince

  2. Faculty of Veterinary Medicine, Department of Biochemistry, Afyon Kocatepe University, TR-03200, Afyonkarahisar, Turkey

    Ismail Kucukkurt

  3. Bayat Vocational School, Department of Laboratory and Veterinary Health, Afyon Kocatepe University, TR-03780, Afyonkarahisar, Turkey

    Hasan Huseyin Demirel & Damla Arslan-Acaroz

  4. Faculty of Medicine, Department of Medical Genetics, Afyonkarahisar Health Science University, TR-03100, Afyonkarahisar, Turkey

    Nuray Varol

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  1. Sinan Ince
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Correspondence to Sinan Ince.

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Ince, S., Kucukkurt, I., Demirel, H.H. et al. Boron, a Trace Mineral, Alleviates Gentamicin-Induced Nephrotoxicity in Rats. Biol Trace Elem Res 195, 515–524 (2020). https://doi.org/10.1007/s12011-019-01875-4

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