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The Effect of Selenium and Polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on Immune Response in Chicken Spleen Under Heat Stress

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Abstract

The aim of the present study was to investigate the effect of selenium (Se), polysaccharide of Atractylodes macrocephala Koidz. (PAMK), and the combination of Se and PAMK on the immune response, heat shock protein (HSP) levels under heat stress (HS) condition in chicken spleen. Two hundred chickens were randomly divided into two groups, the HS group and the control (Con) group. Then these chickens were treated with Se (0.3 mg/kg), PAMK (200 mg/kg) alone, and the combination of Se (0.3 mg/kg) and PAMK (200 mg/kg). The antioxidative enzymes, cytokines contents, and expression levels of HSP27 and HSP70 were examined in chicken spleen. The results indicated that HS induced higher levels of TNF-α, IL-4, HSP27, HSP70, and MDA levels but lower level of IFN-γ, IL-2, Gpx, and SOD in spleen (P < 0.05). These responses were ameliorated by the treatment of Se, PAMK alone, and the combination of Se and PAMK (P < 0.05 or not) The results showed that under common condition, Se and PAMK could improve the immune response by enhancing the levels of some cytokines to proper levels; however, under HS condition, Se and PAMK could change the abnormal levels of cytokines and oxidative damages to ameliorate the injury induced by HS. In addition, there existed synergistic effect on the modulation of these biomarkers in chicken spleen between Se and PAMK. So both Se and PAMK play important roles in regulating the immune function in chicken. Considering the synergistic effect on immune regulation of PAMK, this herb deserves further investigation to evaluate its role in improving the effect of traditional immune regulators.

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Acknowledgments

This research was funded by the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province. The project was supported by Hunan Provincial Natural Science Foundation of China (grant no. 12JJ8014), Zhujiang Science and Technology Star of Guangzhou (grant no. 2011 J2200026), Training Program for Outstanding Young Teachers in Higher Education of Guangdong Province (grant no. Yq2013098), and Key Program of Guangdong Technology Plan (grant no. 2012A020200010).

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Authors and Affiliations

  1. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China

    Danning Xu & Jianhua He

  2. Waterfowl Healthy Breeding Engineering Research Center, Guangdong Higher Education Institutes, Guangzhou, 510225, China

    Danning Xu, Wanyan Li, Yunmao Huang & Yunbo Tian

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  1. Danning Xu
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  2. Wanyan Li
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  3. Yunmao Huang
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  4. Jianhua He
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  5. Yunbo Tian
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Correspondence to Jianhua He or Yunbo Tian.

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Xu, D., Li, W., Huang, Y. et al. The Effect of Selenium and Polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on Immune Response in Chicken Spleen Under Heat Stress. Biol Trace Elem Res 160, 232–237 (2014). https://doi.org/10.1007/s12011-014-0056-y

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  • DOI: https://doi.org/10.1007/s12011-014-0056-y

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