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CeO2 Nanoparticle Bioactive Materials Promote MG-63 Osteogenic Differentiation and Antioxidant Activity Through NRF2 Signaling

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Abstract

The incidence of bone-related diseases is higher in the elderly population, which greatly affects the patients’ quality of life. Throughout this research, we synthesized a biocomposite nanomaterial of CeO2. The unique structural characteristics of CeO2 nanoparticles (CeO2 NPs) were studied by means of XRD, TEM, and SEM. Nanoparticles of an osteosarcoma cell line (MG-63) were assayed for ALP enzyme levels, key proteins in osteoblasts, and stained with Alizarin Red S to assess the physical properties, bioactivity, and calcium deposition of the osteosarcoma cell line. Moreover, we used H2O2 to construct an oxidative stress model to evaluate the antioxidant activity of CeO2 NPs. Experimental data showed that the CeO2 NPs increased the antioxidant capacity of MG-63 cells and significantly increased alkaline phosphatase activity, calcium deposition, and bone growth as manifested by increased expression of bone differentiation proteins BMP2, OCN, OPN, and type I collagen. Interestingly, RNA interference and functional recovery experiments confirmed that CeO2 NPs enhanced the antioxidant activity of MG-63 cells related to NRF2 signaling. In conclusion, the material is expected to be a potential treatment for bone-related diseases.

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Data Availability

Not applicable.

Abbreviations

XRD:

X-ray diffractometer

SEM:

Scanning electron scanning microscope

TEM:

Transmission electron microscope

DLS:

Dynamic light scattering analysis

NRF2:

Red-derived nuclear factor 2-related factor

DRP1:

Dynein-associated protein 1

BAX:

BCL2-associated X

BCL2:

B-cell lymphoma-2

CAS3:

Cancer susceptibility candidate gene 3

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Acknowledgements

We are thankful to Dr. Jun Yan, for critically editing the current manuscript.

Funding

This work is supported by the National Natural Science Foundation of Shandong province (No. ZR202102280280).

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Author notes

  1. Haijuan Liu and Zidong Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Endocrinology, Liaocheng People’s Hospital, Clinical Hospital of Shandong First Medical University, Liaocheng, 252000, China

    Haijuan Liu

  2. Department of Orthopaedic Surgery, Liaocheng People’s Hospital, Clinical Hospital of Shandong First Medical University, Liaocheng, 252000, China

    Zidong Wang, Bei Zhao, Hao Li, Liren Han & Jun Yan

  3. School of Clinical Medicine, Weifang Medical University, Weifang, 261053, China

    Xian Li

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  1. Haijuan Liu
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  5. Hao Li
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The authors contributed equally to this work.

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Correspondence to Liren Han or Jun Yan.

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All experiments in this study have been approved by the Medical Ethics Committee of Liaocheng People’s Hospital with ethics numbers: 2021032 and 2021033.

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Liu, H., Wang, Z., Li, X. et al. CeO2 Nanoparticle Bioactive Materials Promote MG-63 Osteogenic Differentiation and Antioxidant Activity Through NRF2 Signaling. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04766-6

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  • DOI: https://doi.org/10.1007/s12010-023-04766-6

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