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Impact of Acetic Acid Supplementation in Polyhydroxyalkanoates Production by Cupriavidus necator Using Mixture-Process Design and Artificial Neural Network

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Abstract

The trend in bioplastic application has increased over the years where polyhydroxyalkanoates (PHAs) have emerged as a potential candidate with the advantage of being bio-origin, biodegradable, and biocompatible. The present study aims to understand the effect of acetic acid concentration (in combination with sucrose) as a mixture variable and its time of addition (process variable) on PHA production by Cupriavidus necator. The addition of acetic acid at a concentration of 1 g l−1 showed a positive influence on biomass and PHA yield; however, the further increase had a reversal effect. The addition of acetic acid at the time of incubation showed a higher PHA yield, whereas maximum biomass was achieved when acetic acid was added after 48 h. Genetic algorithm (GA) optimized artificial neural network (ANN) was used to model PHA concentration from mixture-process design data. Fitness of the GA-ANN model (R2: 0.935) was superior when compared to the polynomial model (R2: 0.301) from mixture design. Optimization of the ANN model projected 2.691 g l−1 PHA from 7.245 g l−1 acetic acid, 12.756 g l−1 sucrose, and the addition of acetic acid at the time of incubation. Sensitivity analysis indicates the inhibitory effect of all the predictors at higher levels. ANN model can be further used to optimize the variables while extending the bioprocess to fed-batch operation.

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Data Availability

All data generated or analyzed during this study are available from the corresponding author upon reasonable request.

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Acknowledgements

PL and BM acknowledge the support from the Karunya Institute of Technology and Sciences. Standard ethical and professional conduct has been followed.

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    Pema Lhamo & Biswanath Mahanty

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Pema Lhamo contributed to conceptualization-supporting, formal analysis-equal, visualization-lead, writing—original draft-lead, writing—review and editing-equal. Biswanath Mahanty contributed to conceptualization-lead, project administration-lead, supervision-lead, writing—original draft-supporting, writing—review and editing-equal.

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Lhamo, P., Mahanty, B. Impact of Acetic Acid Supplementation in Polyhydroxyalkanoates Production by Cupriavidus necator Using Mixture-Process Design and Artificial Neural Network. Appl Biochem Biotechnol 196, 1155–1174 (2024). https://doi.org/10.1007/s12010-023-04567-x

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