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Co-production of Biohydrogen and Biomethane from Chicken Manure and Food Waste in a Two-Stage Anaerobic Fermentation Process

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Abstract

Batch experiments were performed to evaluate the biohydrogen and biomethane production by co-digestion of chicken manure and food waste in a two-stage mesophilic fermentation process. Results showed that no hydrogen was produced in the first stage of sole chicken manure fermentation, while methane yield was 247.3 mL·g−1-VS. By comparison, the co-digestion process with food waste proportions of 50–85% obtained hydrogen yields of 15.5–57.5 mL·g−1-VS, and the methane yields and maximum specific methane production rates were also improved by 7.0–16.7% and 80%, respectively. Moreover, the highest hydrogen and methane yields were achieved during sole food waste fermentation process. The acetate was the main volatile fatty acid (VFA) produced during sole chicken manure fermentation process in the first stage. Statistical analysis revealed that hydrogen production from co-digestion process and sole food waste fermentation process followed the n-butyrate-type pathway. Meanwhile, it should be noticed that the co-fermentation of chicken manure and food waste had antagonistic effects on the hydrogen fermentation, implying that there might be some inhibition factors existing in chicken manure or produced during the co-fermentation process. At the beginning of methane fermentation, the VFA profiles were similar to those at the end of hydrogen fermentation, and the main VFA compositions changed to acetate and propionate in the latter period of methane production. The volatile solid removal efficiencies were also promoted in co-digestion process compared with sole chicken manure digestion, which were increased by 9.7–14.4% with food waste proportions of 50–80%.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful to the financial support from the National Key R&D Program of China (2018YFD1100503).

Funding

This work was supported by the National Key R&D Program of China (2018YFD1100503).

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Authors and Affiliations

  1. College of Engineering and Technology, Tianjin Agricultural University, Tianjin, 300392, China

    Xinyuan Liu, Yuyu Yang, Nan Wu, Yong Wei, Huiyong Shan & Hui Zhao

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  1. Xinyuan Liu
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  2. Yuyu Yang
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  3. Nan Wu
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  4. Yong Wei
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  6. Hui Zhao
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Contributions

All authors contributed to the study conception and design. Formal analysis and investigation: Xinyuan Liu and Yuyu Yang. Writing—original draft preparation: Xinyuan Liu. Writing—review and editing: Nan Wu. Funding acquisition: Hui Zhao. Supervision: Yong Wei and Huiyong Shan. All authors read and approved the final manuscript.

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Correspondence to Nan Wu.

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Highlights

• Co-digestion of food waste (FW) and chicken manure (CM) produced H2 and CH4.

• The lowest H2 and CH4 yields were obtained at CM proportions of 0–30%.

• Antagonistic effects on H2 fermentation occurred in co-fermentation of FW and CM.

• H2 production from FW and co-substrates followed n-butyrate pathway.

• Digestion efficiencies were enhanced by co-digestion compared to sole CM digestion.

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Liu, X., Yang, Y., Wu, N. et al. Co-production of Biohydrogen and Biomethane from Chicken Manure and Food Waste in a Two-Stage Anaerobic Fermentation Process. Appl Biochem Biotechnol 194, 3706–3720 (2022). https://doi.org/10.1007/s12010-022-03945-1

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