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LncRNA LINC00665 Promotes Ovarian Cancer Cell Proliferation and Inhibits Apoptosis via Targeting miR-181a-5p/FHDC

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Abstract

Previous reports indicate that long intergenic non-coding RNA LINC00665 naturally occurred vital effects in various cancers. Herein, the role of LINC00665 in ovarian cancer progress was explored. We found that LINC00665 was upregulated in ovarian cancer cell lines. Besides, a series of assays including flow cytometry, wound-healing, transwell, cell counting Kit-8 (CCK-8), and EdU assay confirmed that the knockdown of LINC00665 could reduce the viability, proliferation, and migration of SKOV-3 and OVCAR-3 cells. Accumulating evidence indicates that many lncRNAs can function as endogenous miRNA sponges by competitively binding common miRNAs. In this study, the bioinformatics analysis suggests that LNC00665 specifically binds to miR-181a-5p. LINC00665 downregulated the miR-181a-5p in SKOV-3 and OVCAR-3 cells. The knockdown of miR-181a-5p evidently reverses the inhibitory effect of sh-LINC00662. Besides, FH2 domain containing 1 (FHDC1) has been proved to deed as an effective target of miR-181a-5p. The results reveal the knockdown of LINC00665 facilitates ovarian cancer via development by sponging miR-181a-5p and up-regulating FHDC1 expression. These may contribute to ovarian cancer therapy.

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All data generated or analyzed during this study are included in this published article.

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Authors and Affiliations

  1. Department of Women’s Health, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, Jiangsu Province, China

    Suli Wang

  2. Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital (Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital), 42 Baiziting Road, Nanjing, 210009, Jiangsu Province, China

    Suli Wang, Jin Lu & Jinhua Wang

  3. Department of Gynecology, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, Jiangsu Province, China

    Yingchun Wang

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  1. Suli Wang
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  2. Yingchun Wang
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  3. Jin Lu
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  4. Jinhua Wang
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Contributions

S.W conducted most of the experiments. J.W. interpreted and analyzed the data. Y.W wrote the first draft of the article. J.L. finalized the manuscript. J.W. conceived the study and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jinhua Wang.

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Wang, S., Wang, Y., Lu, J. et al. LncRNA LINC00665 Promotes Ovarian Cancer Cell Proliferation and Inhibits Apoptosis via Targeting miR-181a-5p/FHDC. Appl Biochem Biotechnol 194, 3819–3832 (2022). https://doi.org/10.1007/s12010-022-03943-3

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