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Hsa_circ_0023984 Regulates Cell Proliferation, Migration, and Invasion in Esophageal Squamous Cancer via Regulating miR-1294/PI3K/Akt/c-Myc Pathway

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Abstract

Circular RNAs (circRNAs) exert an essential function in the tumorigenesis and progression of esophageal squamous cancer (ESCC). Nonetheless, the role and potential mechanism of circ_0023984 in ESCC are blurred. circRNA expression profile data from Gene Expression Omnibus (GEO) database was applied to analyze circRNAs that were differentially expressed between ESCC tissues and paracancerous tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to analyze circ_0023984 expression and miR-1294 expression in ESCC tissues and cells. Then a series of functional experiments were executed to validate the role of circ_0023984 and miR-1294 in modulating the proliferation, migration, invasion, and cell cycle progression of ESCC cells. Luciferase reporter experiment was performed to confirm the targeting relationship between circ_0023984 and miR-1294. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out with DAVID database. Western blot assay was utilized to detect phosphorylated-Akt (p-Akt) expression and c-Myc expression. circ_0023984 was remarkably upmodulated in ESCC tumor tissues and cell lines. circ_0023984 overexpression was correlated with advanced clinical stage and lymph node metastasis of ESCC patients. circ_0023984 overexpression remarkably enhanced ESCC cell proliferation, migration, invasion, and cell cycle progression, while knockdown of circ_0023984 showed the opposite effect. circ_0023984 was the molecular sponge of miR-1294. miR-1294 could significantly inhibit ESCC cell proliferation, migration, invasion, and induce cell cycle arrest at G0/G1 phase. circ_0023984 affected ESCC progression through regulating miR-1294 expression. The target genes of miR-1294 were associated with cell cycle arrest and PI3K-Akt signaling pathway. circ_0023984 upregulated the expression of p-Akt and c-Myc by repressing miR-1294. circ_0023984 facilitates the malignant biological behaviors of ESCC cells through inhibiting miR-1294 and activating PI3K/Akt/c-myc pathway.

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Data Availability

The data and materials in this study were available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Jianshedong Road No.1, Zhengzhou, 450000, Henan, China

    Wenchang Liang & Mingzhi Zhang

  2. Department of Oncology, Anyang Tumor Hospital, the Affiliated Anyang Tumor Hospital of Henan University of Science and Technology, Henan Medical Key Laboratory of Precise Prevention and Treatment of Esophageal Cancer, Anyang, 455000, Henan, China

    Wenchang Liang, Cailing Wang & Junsheng Wang

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  1. Wenchang Liang
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  2. Cailing Wang
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  3. Junsheng Wang
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  4. Mingzhi Zhang
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Contributions

Wenchang Liang and Mingzhi Zhang conceived and designed the study, and wrote the manuscript; Wenchang Liang, Cailing Wang, and Junsheng Wang collected the data and performed data analysis.

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Correspondence to Mingzhi Zhang.

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Supplementary figure

BEZ235 has no effect on the expression of circ_0023984 and miR-1294 (A-B) qRT-PCR was performed to analyze circ_0023984 or miR-1294 expression in TE-1 and KYSE150 cells treated with BEZ235. ns: P > 0.05 (PNG 126 kb)

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Liang, W., Wang, C., Wang, J. et al. Hsa_circ_0023984 Regulates Cell Proliferation, Migration, and Invasion in Esophageal Squamous Cancer via Regulating miR-1294/PI3K/Akt/c-Myc Pathway. Appl Biochem Biotechnol 194, 1–16 (2022). https://doi.org/10.1007/s12010-022-03935-3

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  • DOI: https://doi.org/10.1007/s12010-022-03935-3

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