Abstract
In this study, nitrilase (Nit) was immobilized in zeolite imidazole framework-90 (ZIF-90) by one-pot biomimetic mineralization strategy. The structure, morphology and functional groups of ZIF-90 and immobilized enzyme Nit@ZIF-90 were characterized by scanning electron microscopy (SEM)/energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR). Circular dichroism (CD) proved that the immobilized method of encapsulation in ZIF-90 could effectively maintain the intrinsic conformation of Nit. Meanwhile, the stability and reusability of Nit@ZIF-90 were systematically evaluated. Compared with the free enzyme, the thermal, pH and organic solvents stability of Nit@ZIF-90 were significantly increased. Further, Nit@ZIF-90 exhibited better reusability during the hydrolysis of acrylonitrile and retained 48.34% of the initial activity after 10 cycles. Besides, the Ni@ZIF-90 had preferable storage stability, which showed a high degree of residual activity (more than 64 %) after storage at 4 °C for 7 d. The improved stability and reusability of the Nit@ZIF-90 implied that it could be used as a potential effective biocatalyst for hydrolysis of nitrile compounds in industrial application.
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This work was financially supported by the National Natural Science Foundation of China (22078019).
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Hui Peng, Yanhong Chang and Hui Luo contributed to the study conception and design. Material preparation, data collection and analysis were performed by Hui Peng, Wenge Dong, Qiwei Chen, Haiyan Song, Hongxu Sun and Ren Li. The first draft of the manuscript was written by Hui Peng, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Peng, H., Dong, W., Chen, Q. et al. Encapsulation of Nitrilase in Zeolitic Imidazolate Framework-90 to Improve Its Stability and Reusability. Appl Biochem Biotechnol 194, 3527–3540 (2022). https://doi.org/10.1007/s12010-022-03890-z
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DOI: https://doi.org/10.1007/s12010-022-03890-z