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Modifying the Microenvironment of Epoxy Resin to Improve the Activity of Immobilized 7α-Hydroxysteroid Dehydrogenases

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Abstract

7α-Hydroxysteroid dehydrogenase (7α-HSDH) is one of the key enzymes in the catalytic reaction of taurochenodeoxycholic acid (TCDCA). To improve the activity of immobilized 7α-HSDH, the microenvironment of immobilized 7α-HSDH was modified with epoxy resin and ethanediamine (EDA). The amino-epoxy support was characterized by Fourier transform infrared (FTIR), Spectrometer elemental analysis (EA), scanning electron microscopy (SEM), contact angle (CA), and Zetasizer. The effects of the immobilization of 7α-HSDH on the amino-epoxy resin and epoxy resin were studied. The results indicated that the relative activity of immobilized 7α-HSDH on the amino-epoxy resin increased by approximately 80%. Meanwhile, the immobilized 7α-HSDH showed favorable thermal stability and operational stability. The thermal stability of immobilized 7α-HSDH increased at temperatures ranging from 15 to 35 °C, while the relative activities of 7α-HSDH immobilized on the amino-epoxy resin and epoxy resin retained 56.4% and 61.0%. After 6 cycles, the residual activities of the 7α-HSDH immobilized on the amino-epoxy resin and epoxy resin were 81.4% and 89.5%, respectively.

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Funding

This work was financially supported by the National Science and Technology Major Projects for “Major New Drugs Innovation and Development” (2017ZX09301306-007) and the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN202001427; KJ1601216).

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  1. Qiong Yang and Liuying Li contributed equally to this work.

Authors and Affiliations

  1. Chongqing Key Laboratory of Inorganic Special Functional Materials, Yangtze Normal University, Chongqing, 408100, People’s Republic of China

    Qiong Yang

  2. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, No. 174, Shapingba Main Street, Chongqing, 400030, People’s Republic of China

    Qiong Yang, Liuying Li, Bochu Wang & Liancai Zhu

  3. Chongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region, School of Biological & Chemical engineering, Chongqing University of Education, Chongqing, 400067, China

    Jun Tan

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  1. Qiong Yang
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Correspondence to Qiong Yang or Bochu Wang.

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Yang, Q., Li, L., Wang, B. et al. Modifying the Microenvironment of Epoxy Resin to Improve the Activity of Immobilized 7α-Hydroxysteroid Dehydrogenases. Appl Biochem Biotechnol 193, 925–939 (2021). https://doi.org/10.1007/s12010-020-03473-w

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