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Enhanced Enzymatic Hydrolysis and Structure Properties of Bamboo by Moderate Two-Step Pretreatment

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Abstract

A moderate two-step pretreatment method was investigated to improve the enzymatic saccharification of bamboo residues. SEM and FTIR were employed to characterize the structure changes. Fed-batch enzymatic saccharification was performed to obtain high concentration of fermentable sugar. Bamboo was impregnated at low severity of conditions (room temperature, 2% H2SO4 or 2% NaOH, 48 h) to initially alter the structure of bamboo, and then further pretreated by steam explosion at 1.0 MPa for 6 min. The highest delignification of 51% and the highest enzymatic hydrolysis of 47.1% were reached at 2% NaOH impregnation followed by steam explosion. The changes in the structural characteristics showed beneficial effects on the enzymatic hydrolysis. When a mixer of cellulase (30 FPU) and β-glucosidase (10 CBU) was further used, the maximum enzymatic hydrolysis of 78.9% and total glucose yield of 68.2% were obtained. The maximum sugar release from the holocellulose was 500 mg/g bamboo, approximately 83.3% conversion efficiency based on monomeric sugar recovery. With fed-batch saccharification, a final substrate loading of 30% brought 107.7 g/L glucose, 35.81 g/L xylose, and 7.82 g/L arabinose release, respectively. This study provided an effective strategy for potential utilization of bamboo residues.

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Funding

The authors wish to express their gratitude for the financial support from the Special Funds for Basic Research Funding for Central public Welfare Scientific Institutes (CAFYBB2014QA023), Open Fund for Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration (JPELBCPI2019001), and National Science-technology Support Plan Projects (2014BAD02B02).

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Authors and Affiliations

  1. Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization; Key and Open Laboratory of Forest Chemical Engineering, SFA; Key Laboratory of Biomass Energy and Material, Nanjing, 210042, Jiangsu Province, People’s Republic of China

    Jing Yang, Hao Xu, Jianchun Jiang, Ning Zhang, Jingcong Xie, Jian Zhao & Min Wei

  2. Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Jing Yang, Hao Xu, Jianchun Jiang, Ning Zhang, Jingcong Xie, Jian Zhao & Min Wei

  3. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, 223003, People’s Republic of China

    Jing Yang

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  1. Jing Yang
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  2. Hao Xu
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  3. Jianchun Jiang
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Correspondence to Jianchun Jiang.

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Yang, J., Xu, H., Jiang, J. et al. Enhanced Enzymatic Hydrolysis and Structure Properties of Bamboo by Moderate Two-Step Pretreatment. Appl Biochem Biotechnol 193, 1011–1022 (2021). https://doi.org/10.1007/s12010-020-03472-x

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