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The Influence of Submerged Fermentation of Inonotus obliquus with Control Atmosphere Treatment on Enhancing Bioactive Ingredient Contents

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Abstract

This study led in the pioneering technique incubated in a bioreactor with the forced air injection system. The purpose of this study was to establish the optimal incubation conditions for this technique. The results showed that the speed at which Inonotus obliquus was incubated with the forced air injection system was superior to that with a normal bioreactor. A nitrogen to oxygen ratio of 50:50 provided the best results with the forced air injection system, including in terms of the achievement of biomass, total triterpenes, betulinic acid content, and the scavenging activities of DPPH radicals, which reached up to 21.3 g/1000 mL, 2.1 g/1000 mL, 1.9 g/1000 mL, and 87.3%, respectively. The results showed that the bioreactor with the forced air injection system could more effectively incubate I. obliquus by using less vapor while still utilizing a model close to that of a traditional bioreactor. The innovative bioreactor fermentation model was thus more economical than the traditional bioreactor model.

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Funding

The authors thank the financial support for the Ministry of Science and Technology, Taiwan, ROC (MOST104-2262-E-241-004-CC3).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd. South District, Taichung, Taiwan, Republic of China

    Hsin-Jung Chen & Chin-Shuh Chen

  2. Department of Food Science & Technology, Central Taiwan University of Science and Technology, Beitun District, Taichung, Taiwan, Republic of China

    Hsin-Jung Chen & Bo-Kang Liou

  3. Department of Food Science and Technology, HungKuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung, Taiwan, Republic of China

    Yuh-Shuen Chen & Shih-Lun Liu

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  1. Hsin-Jung Chen
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Correspondence to Yuh-Shuen Chen.

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Chen, HJ., Chen, YS., Liu, SL. et al. The Influence of Submerged Fermentation of Inonotus obliquus with Control Atmosphere Treatment on Enhancing Bioactive Ingredient Contents. Appl Biochem Biotechnol 191, 412–425 (2020). https://doi.org/10.1007/s12010-020-03273-2

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