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Metal-Organic Frameworks Conjugated Lipase with Enhanced Bio-catalytic Activity and Stability

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Abstract

Covalent immobilization of lipase onto a solid carrier is an effective way to enhance stability. Immobilization inhibits the activity of lipase due to decreased flexibility of enzyme structure via the covalent bond. In this study, monomer of the metal-organic frameworks (MOFs) material ZIF-8 (2-methyl imidazole-4-carboxylic acid) was innovatively used as a chemical modifier of Candida nrugosa lipase (CRL). The circular dichroism spectra results show that the CRL molecule was altered by chemical modification and thus its catalytic activity was 1.3 times higher than that of the free CRL. The modified CRL molecule was further immobilized in the “skeleton” of ZIF-8 through the monomer while in situ forming the cell skeleton of the MOFs, which prevent the active center from being destroyed. The results show that conjugation of chemical modification and immobilized enzymes ensure that there was no obvious reduction in the activity of CRL after immobilization and the stability of CRL was improved. Especially, the organic solvent stability of the modified immobilization CRL in isopropanol was significantly improved and retained more than 148% of its activity.

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Acknowledgments

The work was funded by the National Natural Science Foundation of China (No. 21406093), Natural Science Foundation of Jiangsu Province (BK20140529), Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University (SKLF-KF-201919), Key University Science Research Project of Jiangsu Province (14KJB530001), China Postdoctoral Science Foundation (2014 M550271), and Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Bin Zou, Liming Zhang, Pengyun Wang, Yan Yan, Xinyi Wang & Idowu Onyinye Adesanya

  2. School of Chemistry and Chemical Engineering, Jiangsu University, No.301 Xuefu Road, Zhenjiang, 212013, China

    Jiaojiao Xia

  3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Bin Zou & Jiaojiao Xia

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  1. Bin Zou
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  2. Liming Zhang
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Zou, B., Zhang, L., Xia, J. et al. Metal-Organic Frameworks Conjugated Lipase with Enhanced Bio-catalytic Activity and Stability. Appl Biochem Biotechnol 192, 132–145 (2020). https://doi.org/10.1007/s12010-020-03268-z

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