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Chitosan-Based Surface Molecularly Imprinted Polymer Microspheres for Sustained Release of Sinomenine Hydrochloride in Aqueous Media

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Abstract

The surface molecular imprinting technique has been proposed as a prospective strategy for template molecule recognition and separation by devising the recognition sites on the surface of imprinted materials. The purpose of this study was to establish a novel drug delivery system which was developed by surface molecular imprinting method using β-cyclodextrin (β-CD)-grafted chitosan (CS) (CS-g-β-CD) microspheres as matrix and sinomenine hydrochloride (SM) as the template molecule. By adjusting the amount of functional monomer and cross-linking agent, we got the more excellent adsorption of CS-g-β-CD molecularly imprinted polymers (MIPs-CS-g-β-CD). When the amount of functional monomer was 6 mmol and cross-linking agent was 20 mmol, the maximum binding capacity of MIPs and non-imprinted polymers (NIPs) was 55.9 mg/g and 37.2 mg/g, respectively. The results indicated that the recognition of SM with MIPs was superior to NIPs. The adsorption isotherms of MIPs-CS-g-β-CD indicated that the adsorption behavior fitted better to the Langmuir model, which showed that the adsorption process of polymer was monomolecular layer. In in vitro drug release studies, the accumulative release amount of MIPs-CS-g-β-CD was up to 78% within 24 h. MIPs exhibited an excellent controlled SM release profile without burst release and the mechanism of SM release was shown to conform to non-Fick diffusion. Therefore, MIPs-CS-g-β-CD were successfully applied to extraction of SM and used as the materials for drug delivery system.

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Funding

This work was supported by the Tianjin City High School Science & Technology Fund Planning Project (No. 20140305) and Tianjin Science and Technology Major Projects (No. 16ZXMJSY00130).

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  1. Hanqiu Chen and Wen Zhang contributed equally to this work.

Authors and Affiliations

  1. Tianjin Municipal Key Lab of Advanced Fiber and Energy Storage, School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin, 300387, China

    Hanqiu Chen, Wen Zhang, Chongmin Chen & Mingliang Zhang

  2. Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Pingjin Hospital Heart Center, Logistics University of PAPF, Tianjin, 300162, China

    Ning Yang

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  1. Hanqiu Chen
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Correspondence to Wen Zhang.

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Chen, H., Zhang, W., Yang, N. et al. Chitosan-Based Surface Molecularly Imprinted Polymer Microspheres for Sustained Release of Sinomenine Hydrochloride in Aqueous Media. Appl Biochem Biotechnol 185, 370–384 (2018). https://doi.org/10.1007/s12010-017-2658-2

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