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Generation of a Floxed Allele of the Mouse MicroRNA-200 Clusters

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Abstract

MicroRNA-8 and its target gene, u-shaped (ush), regulate body size in Drosophila. As the mouse homolog of the Drosophila miR-8, whether the miR-200 family has similar functions and how they perform their regulatory roles in body size control is unknown. In order to discover the biological function of the miR-200s in vivo, we generated mice lacking the miR-200b/200a/429 gene cluster using the Cre/loxp system. miR-200b/200a/429 null mutant mice were viable, fertile, and showed normal development.

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Acknowledgements

We thank Gaoxiang’s laboratory of the Model Animal Research Center of Nanjing University for their support in generating the loxp-floxed mice.

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Authors and Affiliations

  1. Key Laboratory of Animal Embryo & Molecular Breeding of Hubei Province, Institute of Veterinary and Animal Science, Hubei Academy of Agricultural Science, Wuhan, 430064, China

    Hongyan Ren, Yanzhen Bi & Xinmin Zheng

  2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Cong Tao & Kui Li

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  1. Hongyan Ren
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  2. Cong Tao
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  3. Kui Li
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  4. Yanzhen Bi
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  5. Xinmin Zheng
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Corresponding author

Correspondence to Xinmin Zheng.

Ethics declarations

This study was performed in accordance with the Guidelines of Animal Experimentation at the Institutional Animal Care Research Advisory Committee of the Chinese Academy of Agricultural Sciences (CAAS, Beijing, China). All animal experiments were performed in accordance with the regulations of the animal care and use committee of the model animal research center.

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Ren, H., Tao, C., Li, K. et al. Generation of a Floxed Allele of the Mouse MicroRNA-200 Clusters. Appl Biochem Biotechnol 182, 1218–1228 (2017). https://doi.org/10.1007/s12010-016-2394-z

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  • DOI: https://doi.org/10.1007/s12010-016-2394-z

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