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Inhibition of Tumor Necrosis Factor-α and Interleukin-1β Production in Lipopolysaccharide-Stimulated Monocytes by Methanolic Extract of Elephantopus scaber Linn and Identification of Bioactive Components

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Abstract

The methanolic extract of E. scaber Linn was evaluated for anti-inflammatory activity by determining its effects on production of pro-inflammatory cytokines like Tumor necrosis factor-α (TNF-α) and Interleukin-1β (IL-1β) in Lipopolysaccharide (LPS) stimulated monocytes. The cytotoxicity of the extract was analyzed prior to the cytokine quantification assays. The extract was further subjected to UPLC MS Q-TOF, for the identification of bioactive components present in the crude extract. The extract was found not to be cytotoxic against monocytes, and exhibited significant inhibition in the production of pro-inflammatory cytokines. The presence of 34 components in the methanolic extract was detected through mass spectrum analysis.

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Acknowledgments

The authors would like to thank the Kerala State Council for Science Technology and Environment (086/2012/KSCSTE/DATED01/02/2012) for financial support and to DBT MSUB (BT/PR4800/INF/22/152/2012 dated 22/03/2012) for their instrumentation support. The authors thank Dineep Devadasan (School of Environmental Science, Mahatma Gandhi University, Kottayam, Kerala) for his assistance with the UPLC MS Q-TOF analysis.

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  1. Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India, 686560

    Anu P. Abhimannue, Mohind C. Mohan & Prakash Kumar B

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  1. Anu P. Abhimannue
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  2. Mohind C. Mohan
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  3. Prakash Kumar B
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Correspondence to Prakash Kumar B.

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Abhimannue, A.P., Mohan, M.C. & B, P.K. Inhibition of Tumor Necrosis Factor-α and Interleukin-1β Production in Lipopolysaccharide-Stimulated Monocytes by Methanolic Extract of Elephantopus scaber Linn and Identification of Bioactive Components. Appl Biochem Biotechnol 179, 427–443 (2016). https://doi.org/10.1007/s12010-016-2004-0

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