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The Preparation and Ethanol Fermentation of High-Concentration Sugars from Steam-Explosion Corn Stover

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Abstract

In the field of biofuel ethanol, high-concentration- reducing sugars made from cellulosic materials lay the foundation for high-concentration ethanol fermentation. In this study, corn stover was pre-treated in a process combining chemical methods and steam explosion; the cellulosic hydrolyzed sugars obtained by fed-batch saccharification were then used as the carbon source for high-concentration ethanol fermentation. Saccharomyces cerevisiae 1308, Angel yeast, and Issatchenkia orientalis were shake-cultured with Pachysolen tannophilus P-01 for fermentation. Results implied that the ethanol yields from the three types of mixed strains were 4.85 g/100 mL, 4.57 g/100 mL, and 5.02 g/100 mL (separately) at yield rates of 91.6, 89.3, and 92.2 %, respectively. Therefore, it was inferred that shock-fermentation using mixed strains achieved a higher ethanol yield at a greater rate in a shorter fermentation period. This study provided a theoretical basis and technical guidance for the fermentation of industrial high-concentrated cellulosic ethanol.

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Acknowledgments

This research was financially supported by National High Technology Research and Development Program (863 Program) in Biology and Medicine Technology Field of China (No. 2012AA022301B) and the Science and Technology Cooperation Project between Henan Province and Academy of China (No. 102106000018).

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Authors and Affiliations

  1. Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Nongye Road No. 63, Zhengzhou, 450002, China

    Hui Xie, Fengqin Wang, Shuangyao Yin & Andong Song

  2. National Tobacco Cultivation and Physiological and Biochemical Research Centre, Henan Agricultural University, Zhengzhou, 450002, China

    Tianbao Ren

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  1. Hui Xie
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  2. Fengqin Wang
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  3. Shuangyao Yin
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  4. Tianbao Ren
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  5. Andong Song
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Correspondence to Andong Song.

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Xie, H., Wang, F., Yin, S. et al. The Preparation and Ethanol Fermentation of High-Concentration Sugars from Steam-Explosion Corn Stover. Appl Biochem Biotechnol 176, 613–624 (2015). https://doi.org/10.1007/s12010-015-1599-x

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